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目的通过2014-2015年内第一次检测艾滋病病毒载量出现无效结果的标本,进行第二次检测,所得病毒载量值与同批量检测标本进行比较,统计是否存在差异、是否存在相关性和一致性。方法收集同年内所有HIV感染者抗凝血的无效标本,13 000 g离心2 min,应用HIV-1核酸序列扩增技术检测各无效病毒载量标本,并且在统一时间内对其他标本进行检测,对两次检测结果进行比较。结果对于两次所检测的标本结果差别不大,无统计学意义。结论对于病毒载量标本反复冻融一次,其检测结果变化不大,运送HIV病人的外周血进行病毒载量检测时候,应尽量低温并且以血浆的形式保存,血浆严格按照检测技术规范的要求进行离心,保存。
Objective To test for the second time between 2014-2015 when the first test of HIV-infected patients showed invalid results and compare the results with those of the same test to determine whether there is a difference and whether there is correlation and consistency Sex. Methods The samples of all HIV-infected patients were collected and centrifuged at 13 000 g for 2 min in each year. HIV-1 nucleic acid sequence amplification was used to detect each invalid viral load, and other samples were tested at a uniform time. The two test results were compared. Results for the two specimens tested the difference is not significant, not statistically significant. Conclusions The results showed little change after repeated freeze-thaw cycles of virus-loaded specimens. When the viral load was detected in the peripheral blood of HIV-infected patients, they should be kept as low temperature as possible and preserved in plasma. The plasma should be in strict accordance with the requirements of the test specification Centrifugal, save.