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目的探讨青海高原地区子痫前期患者胎盘组织及血清中人类白细胞相关抗原G(HLA-G)蛋白表达研究及临床意义。方法用蛋白印迹法(Western blot)检测30例正常晚期妊娠妇女及30例子痫前期患者(轻度15例,重度15例)的胎盘组织中HLA-G蛋白的表达差异;并应用双抗体夹心酶联免疫吸附法(ELISA)检测血清中可溶性HLA-G(s HLA-G)的表达。结果青海高原地区重度子痫前期与轻度子痫前期患者胎盘组织中HLA-G蛋白表达水平均明显低于正常妊娠组,两者比较,差异有统计学意义(P<0.01)。轻重度之间差异亦有统计学意义(P<0.01)。重度子痫前期与轻度子痫前期患者血清中s HLA-G浓度均低于正常足月妊娠组,差异有统计学意义(P<0.01),轻重度之间差异亦有统计学意义(P<0.01)。子痫前期患者血清中s HLA-G浓度与胎盘组织HLA-G蛋白表达水平呈正相关(r=0.883,P<0.01)。结论青海高原地区子痫前期患者血清及胎盘中HLA-G蛋白表达均下降,可能与子痫前期的发生及疾病的轻重程度相关,血清s HLA-G拟可作为子痫前期的早期诊断的指标。
Objective To investigate the expression of human leukocyte antigen-G (HLA-G) in placenta and serum of patients with preeclampsia in Qinghai Plateau and its clinical significance. Methods Western blot was used to detect the expression of HLA-G protein in placentas of 30 normal pregnant women and 30 pregnant women with preeclampsia (15 mild and 15 severe) Serum soluble HLA-G (s HLA-G) expression was detected by ELISA. Results The levels of HLA-G protein in placenta of patients with severe preeclampsia and mild preeclampsia in Qinghai Plateau were significantly lower than those in normal pregnancy group (P <0.01). There was also a significant difference between severity and severity (P <0.01). Serum levels of HLA-G in patients with severe preeclampsia and mild preeclampsia were lower than those of normal term pregnancy (P <0.01), and there was also a significant difference between the two groups <0.01). Serum levels of HLA-G in preeclampsia were positively correlated with HLA-G protein expression in placenta (r = 0.883, P <0.01). Conclusion The expression of HLA-G protein in serum and placenta of preeclampsia patients in Qinghai Plateau is decreased, which may be related to the occurrence of preeclampsia and severity of disease. Serum s HLA-G may be used as an indicator of early diagnosis of preeclampsia .