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目的探讨正常孕妇和妊娠期糖尿病(GDM)孕妇血清和胎盘中尾加压素Ⅱ(UⅡ)的表达及其临床意义。方法选取2012年6月~2013年12月在该院正规产检并住院分娩的孕妇80例,其中正常孕妇40例(对照组),GDM孕妇40例(观察组)。采用酶联免疫吸附试验(ELISA)法检测两组孕妇外周血血清中的UⅡ水平;采用RT-PCR观察胎盘中UⅡmRNA的表达。结果两组孕妇血清中UⅡ水平值比较,差异有统计学意义(t=2.32,P<0.05);两组孕妇胎盘组织中UⅡmRNA表达水平比较,差异有统计学意义(t=1.67,P<0.05);两组血清UⅡ水平与胎盘组织UⅡmRNA表达水平均呈正相关(r=0.73,P<0.05;r=0.72,P<0.05)。结论 UⅡ在GDM孕妇血清及胎盘中的高表达提示其参与了GDM的发生与发展。
Objective To investigate the expression of urotensin Ⅱ (UⅡ) in serum and placenta of pregnant and gestational diabetes mellitus (GDM) pregnant women and its clinical significance. Methods Totally 80 pregnant women undergoing regular birth control and hospital delivery in our hospital from June 2012 to December 2013 were selected, including 40 normal pregnant women (control group) and 40 pregnant women with GDM (observation group). The level of UⅡin peripheral blood serum of two groups was detected by enzyme linked immunosorbent assay (ELISA). The expression of UⅡmRNA in placenta was observed by RT-PCR. Results There was significant difference in serum UⅡ levels between the two groups (t = 2.32, P <0.05). The levels of UⅡmRNA in placenta between the two groups were significantly different (t = 1.67, P <0.05 ). There was a positive correlation between serum UⅡ level and placental UⅡmRNA expression level (r = 0.73, P <0.05; r = 0.72, P <0.05). Conclusion The high expression of UⅡ in serum and placenta of pregnant women with GDM suggests that it is involved in the occurrence and development of GDM.