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目的从艾滋病病毒（HIV）感染者血液中分离HIV，进行HIV分离株的生物学特征研究。方法采集10份福建艾滋病病毒感染者肝素抗凝血，分离外周血单核细胞（PBMC），与健康人PBMC共培养进行HIV-1的分离，使用含神经氨酸酶（NA）的T细胞培养液提高病毒分离率，通过检测F24抗原、间接免疫荧光试验（IFA）及电镜观察等确定病原分离结果。用H9及MT4细胞对分离的病毒进行细胞嗜性的研究。结果从10例病例的PBMC标本中分离到9株HIV-1，分离率达90％。9株病毒健康株均可感染MT4细胞，引起细胞融合，但8株病毒仅表现为对MT4的一过性感染，只有FJ113分离株表现为M14细胞的持续性感染，传至15代，P24抗原检测OD值仍无明显变化，确定为快／高病毒分离株。结论本方法HIV分离率高，福建病毒分离株主要为M嗜性。
Objective To isolate HIV from the blood of people living with HIV and to study the biological characteristics of HIV isolates. Methods Heparin anticoagulant blood was collected from 10 HIV-infected persons in Fujian province. Peripheral blood mononuclear cells (PBMCs) were isolated and co-cultured with PBMCs of healthy people for HIV-1 isolation. T-cell cultures containing neuraminidase (NA) Liquid to improve the isolation rate of virus. F24 antigen was detected by indirect immunofluorescence assay (IFA) and electron microscopy to determine the pathogen isolation results. The cytotoxicity of the isolated virus was studied using H9 and MT4 cells. Results Nine strains of HIV-1 were isolated from PBMC specimens of 10 cases and the isolation rate was 90%. All of the 9 strains of virus could infect MT4 cells and cause cell fusion. However, 8 strains of virus showed only transient infection of MT4. Only FJ113 isolates showed persistent infection of M14 cells and passed on to passage 15, P24 antigen Detection of OD values still no significant change, identified as fast / high virus isolates. Conclusion The HIV isolation rate of this method is high, Fujian virus isolates mainly M tropism.