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PLC/PRF/5株人类肝癌细胞含有整合的乙肝病毒DNA,且能合成和分泌乙肝病毒表面抗原(HB_sAg)。当注射给BALB/C系无胸腺裸鼠,几乎100%诱发肿瘤产生。用免疫荧光技术研究已表明HB_sAg存在于PLC/PRF/5细胞表面及胞浆内。本文报告抗-HBs单克隆抗体IgM和IgG_(2a)能预防或抑制接受PLC/PRF/5细胞注射的无胸腺裸鼠的肿瘤形成。将10~7PLC/PRF/5细胞经皮下注射给4～6周龄的雄性BALB/C裸鼠,在一小时内再经静脉注射100μl抗-HBs单克隆抗体IgM或经腹腔注射100μl抗-HBs单克隆IgG_(2a)、IgG_1;对照组注射100μl PBS液。以后28～32天内隔天反复注射。结果表明:对照组肿
The PLC / PRF / 5 human hepatoma cells contain integrated hepatitis B virus DNA and are capable of synthesizing and secreting hepatitis B virus surface antigen (HB_sAg). When injected into BALB / c athymic nude mice, almost 100% of the tumors were induced. Immunofluorescence studies have shown HBsAg exists in the surface of PLC / PRF / 5 cells and cytoplasm. The present report reports that anti-HBs monoclonal antibodies IgM and IgG2 (2a) prevent or inhibit tumor formation in athymic nude mice receiving PLC / PRF / 5 cell injections. Male BALB / C nude mice, 4 to 6 weeks of age, were subcutaneously injected with 10-7 PLC / PRF / 5 cells and intravenously injected with 100 μl of anti-HBs monoclonal antibody IgM or 100 μl of anti-HBs Monoclonal IgG_ (2a), IgG_1; control group was injected with 100μl PBS solution. After 28 to 32 days after repeated injections. The results showed that the control group swollen