论文部分内容阅读
被称为BK病毒的新乳多泡病毒(Papovavirug)是Gardner等由肾脏移植患者尿中分离的。后又从患急性骨髓单核细胞性白血病、急性骨髓性白血病及精原细胞瘤等疾病的患者及孕妇中也分离到。此种病毒繁殖慢,细胞亲和力强,而且不引起明显的细胞病变,所以分离培养十分困难。著者就BK病毒感染的快速诊断的可能性进行了研究。采取SLE患者(M·T)晨尿150ml,1000转低速离心10分钟,收集沉渣加入2ml Hank’s液悬浮,做为“细胞”样品,将离心上清再经5000转30分钟离心,取其上清(约120ml)再经30000转高速离心2小时,收集沉渣悬浮于1.5ml Hank’s液内,做为“沉淀”样品。取“细胞”样品一部分做成涂抹标本,用HE染色和荧光抗体染色,另一部分用超声波处理,(20KH3分钟)破坏细胞用于病毒分离。具体方法:接种0.1ml标本于HEL—R66细胞,室温吸附60分钟后加入含Amphotericin B5μg/ml的0.5%小牛血清MEM培养液1ml,在37℃培养。
Papovavirug, known as the BK virus, is isolated from the urine of kidney transplant patients by Gardner et al. Later, patients with acute myelomonocytic leukemia, acute myelogenous leukemia and seminoma and other diseases and pregnant women were also isolated. The virus is slow to reproduce, strong cell affinity, and does not cause significant cytopathic effect, so the isolation and culture is very difficult. The authors studied the possibility of rapid diagnosis of BK virus infection. Take 150 ml of morning urine of SLE patients (M · T) and centrifuge at low speed for 1000 minutes for 10 minutes. Collect the sediment and add 2 ml Hank’s liquid suspension to make a “cell” sample. Centrifuge the supernatant and centrifuge at 5000 rpm for 30 minutes. The supernatant (about 120 ml) was centrifuged at 30,000 rpm for 2 hours. The pellet was collected and suspended in 1.5 ml Hank’s solution as a “pellet” sample. Part of the “cell” sample was smear-stained, stained with HE staining and fluorescent antibody, and the other part was treated with ultrasound (20KH for 3 minutes) to destroy cells for virus isolation. Specific method: Inoculate 0.1ml specimen in HEL-R66 cells, adsorb 60 minutes at room temperature, then add 1ml of 0.5% fetal bovine serum MEM containing Amphotericin B 5μg / ml and incubate at 37 ℃.