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为了澄清肥大细胞在分泌性中耳炎（SOM）病因学中的作用，采用聚丙烯酸胺梯度凝胶电泳及等电聚焦电泳法比较了中耳渗液（MEE）及人肥大细胞的蛋白成分。文中详细的介绍了所采用的改进的人肥大细胞组织培养方法。①用脾细胞制备的条件培养基：自一患有球形红细胞症8岁患儿切除的
To elucidate the role of mast cells in the etiology of secretory otitis media (SOM), polyacrylamide gel electrophoresis and isoelectric focusing were used to compare the protein profiles of the middle ear effusion (MEE) and human mast cells. The paper introduces in detail the improved method of human mast cell culture. ① Conditioned medium prepared with spleen cells: excised from an 8-year-old child with spherocytosis