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纤维连结蛋白(Fibronectin FN)是一种大分子粘连性糖蛋白,通过几种间接和直接免疫组织学方法观察到在正常肾小球中,突出地定位于肾小球系膜。因而使FN成为肾小球系膜标志的一个特征。另一方面,在膜性肾病中观察FN呈弥漫性毛细血管性分布,而其系膜增生不太突出。根据生化研究,这些结果提示在病变的肾,FN可能部分起源于系膜或密切地与4型胶原或纤维蛋白同时存在。为确定FN的精确定位,我们用异硫氰荧光光素(FITC)或四甲基硷性蕊香红异硫氰酸盐(TRITC)结合特异性抗体,应用直接的或双重免疫荧光显微镜(IF)检查人肾病例活
Fibronectin FN is a macromolecule conglutinating glycoprotein that is prominently localized in the glomerular mesangium in normal glomeruli by several indirect and direct immunohistological methods. Thus making FN a hallmark of the glomerular mesangial marker. On the other hand, FN showed diffuse capillary distribution in membranous nephropathy, and mesangial hyperplasia was not prominent. According to biochemical studies, these results suggest that in diseased kidneys, FN may originate partially from mesangial or closely coexist with type 4 collagen or fibrin. In order to determine the precise localization of FN, we used specific antibodies against isothiocyanatofluorescein (FITC) or tetramethyltrichinanisothiocyanate (TRITC) by direct or double immunofluorescence microscopy (IF ) Check the human kidney cases live