论文部分内容阅读
目的 探讨慢性间断性缺氧后细胞色素C的释放、 caspase-3 的激活以及维生素 E 和左旋丁基苯酞(L-NBP)对这些变化的影响。 方法 建立大鼠慢性间断性缺氧模型,并给予 L-NBP 和维生素E 。用 W estern blot方法和免疫组化方法分别检测大鼠皮层的细胞色素 C 释放和 caspase-3 的激活。 结果 对照组可检测到微弱的caspase-3 阳性染色。缺氧组 caspase蛳3 阳性染色显著增加(P < 0.01)。与缺氧组相比,大、小剂量维生素 E 组,大、小剂量L-NBP 组阳性细胞均明显减少( < 0.01)。对照组线粒体可见明显的细胞色素 C 蛋白条带,而胞浆则无此条 P带。缺氧组胞浆可见该蛋白表达, 而线粒体蛋白条带的表达则相应降低。与缺氧组相比大 小剂量维生素 以及 E大、小剂量 L-NBP 均可明显抑制线粒体细胞色素 C 的释放, 有非常显著性差异( P < 0.01)。 结论 慢性间断性缺氧能够诱导细胞色素C由线粒体至胞浆的释放和caspase-3 的激活。维生素 E 和 L-NBP 能够降低细胞色素C的释放及 caspase-3 的裂解激活。
Objective To investigate the effects of chronic intermittent hypoxia on cytochrome C release, caspase-3 activation, and vitamin E and L-NBP on these changes. Methods Chronic intermittent hypoxia model was established in rats, and L-NBP and vitamin E were given. Western blotting and immunohistochemistry were used to detect the release of cytochrome C and the activation of caspase-3 in rat cortex. Results In the control group, weak caspase-3 positive staining was detected. The hypoxia group caspase 蛳 3 positive staining increased significantly (P <0.01). Compared with hypoxia group, the positive cells in large and small doses of vitamin E group, large and small doses of L-NBP group were significantly decreased (P <0.01). The control group mitochondria obvious cytochrome C protein band, while the cytoplasm does not have this P band. The protein expression was observed in the cytoplasm of hypoxia group, while the expression of mitochondrial protein band was reduced accordingly. Compared with hypoxia group, the dose of vitamin D and the small dose of L-NBP significantly inhibited the release of mitochondrial cytochrome C, with significant difference (P <0.01). Conclusion Chronic intermittent hypoxia can induce the release of cytochrome C from mitochondria to cytoplasm and the activation of caspase-3. Vitamin E and L-NBP reduce cytochrome C release and caspase-3 cleavage activation.