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Aim:To investigate effect of the nociceptin/orphanin FQ(OFQ)on hypothala-mus gonadotropin-releasing hormone(GnRH)release in ovariectomized(OVX)rats.Methods:GnRH radioimmunoassay(RIA)was used to study the effect ofOFQ on GnRH release in hypothalamus slices in vitro.Push-pull perfusion andintracerebroventicular(icy)injection were used to examine the effect of OFQ onGnRH release in the hypothalamus medial preoptic area(POA)in vivo.Ovariecto-mies were performed on female Sprague-Dawley rats,and their plasma luteinizinghormone(LH)levels were measured after icy injection of OFQ with or without[Nphe~1]NC(1-13)NH_2,a competitive antagonist of opioid receptor-like 1 receptor(ORL1 receptor).Reverse transcription-polymerase chain reaction(RT-PCR)wasused to investigate the expression of the ORLI receptor in rat pituitary.Results:GnRH release from hypothalamus slices was inhibited 90 min after the administra-tion of 2 mmol/L and 20 mmol/L OFQ(P<0.05).Accordingly,GnRH release fromhypothalamus POA was also significantly reduced by the injection of 0.2 mmol/Land 2 mmol/L OFQ.Plasma LH levels were also decreased significantly 2 h aftericv injection of 20 nmol OFQ in OVX rats(P<0.05)and this effect could be abol-ished by pretreatment with 20 nmol [Nphe~1]NC(1-13)NH2,that is,NC 13.Moreinterestingly,plasma LH levels in OVX rats increased markedly 2 h after icv injec-tion of 100 nmol and 200 nmol NC 13.RT-PCR analysis further revealed that theORL1 receptor was not expressed in the pituitary of OVX rats.Conclusion:Cen-tral administration of nociceptin/orphanin FQ might inhibit the release of hypo-thalamic GnRH and decrease the plasma LH levels through ORLI receptors inOVX rats.
Aim: To investigate effect of the nociceptin / orphanin FQ (OFQ) on hypothala-mus gonadotropin-releasing hormone (GnRH) release in ovariectomized (OVX) rats. Methods: GnRH radioimmunoassay (RIA) was used to study the effect of OFQ on GnRH release in hypothalamus slices in vitro. Push-pull perfusion and intratrachebrovascular (icy) injection were used to examine the effect of OFQ on GnRH release in the hypothalamus medial preoptic area (POA) in vivo. Ovariecto-mies were performed on female Sprague-Dawley rats, and Their plasma luteinizinghormone (LH) levels were measured after icy injection of OFQ with or without [Nphe ~ 1] NC (1-13) NH_2, a competitive antagonist of opioid receptor-like 1 receptor (ORL1 receptor) reaction (RT-PCR) was used to investigate the expression of the ORLI receptor in rat pituitary. Results: GnRH release from hypothalamus slices was inhibited for 90 min after the administration of 2 mmol / L and 20 mmol / L OFQ (P <0.05 ). According to, GnRH release from hyphothalamus POA wa s also significantly reduced by the injection of 0.2 mmol / Land 2 mmol / L OFQ.Plasma LH levels were also significantly decreased 2 h aftericv injection of 20 nmol OFQ in OVX rats (P <0.05) and this effect could be abol-ished by pretreatment with 20 nmol [Nphe ~ 1] NC (1-13) NH2, that is, NC 13. Murphy Interestingly, plasma LH levels in OVX rats increased markedly after 2 h after icv injection in 100 nmol and 200 nmol NC 13. RT -PCR analysis further revealed that the ORL1 receptor was not expressed in the pituitary of OVX rats. Confc: Cen-tral administration of nociceptin / or phanin FQ might inhibit the release of hypo-thalamic GnRH and decrease the plasma LH levels through ORLI receptors in OVX rats.