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Aim:To determine the effects of acetamide-45 on respiratory function,airwayinflammation,and the activity of phosphodiesterase 4(PDE4)in allergic rats.Methods:Rats were sensitized by a single intramuscular injection with ovalbumin(OVA)and were challenged with ovalbumin applied by using an aerosol repeat-edly for 7 d after 2 weeks.Acetamide-45 at concentrations of 5,10,or 30 mg/kg wasthen administered by intraperitoneal injection.Changes in dynamic lung compli-ance and lung resistance,the accumulation of inflammatory cells in bronchoalveolarlavage,PDE4 activity,and the concentration of interleukin-4 in rat lung tissuewere determined.Results:Seven days of treatment with acetamide-45 preventedeosinophil accumulation in allergic rats.At doses of 5,10,and 30 mg/kg,aceta-mide-45 decreased lung resistance to 0.20±0.04,0.25±0.07,and 0.22±0.05cmH_2O·s~(-1)·mL~(-1),respectively(P<0.05 vs OVA),and it also increased dynamic lungcompliance to 0.41±0.07,0.39±0.06,and 0.42±0.09 mL/cmH20(P<0.05 vs OVA).After being treated with different doses of acetamide-45,the PDE4 activities inlung tissue were 281±55,273±57,and 238±36 nmol·g~(-1)·min~(-1)(P<0.05 vs OVA),andthe concentrations of interleukin-4 in lung tissue were 6.22±1.13,5.95±1.20,and 5.68±2.20 μtg/g protein(P<0.05 vs OVA).Conclusions:Acetamide-45 wasfound to improve respiratory function and inhibit airway inflammation in thisanimal model,and the PDE4 activity of lung tissue was obviously inhibited.Acetamide-45 was an effective anti-inflammatory agent in respiratory inflammation,and the mechanism of its action might depend on inhibition of PDE4.
Aim: To determine the effects of acetamide-45 on respiratory function, airway inflammation, and activity of phosphodiesterase 4 (PDE4) in allergic rats. Methods: Rats were sensitized by a single intramuscular injection with ovalbumin (OVA) and were challenged with ovalbumin applied by using an aerosol repeat-edly for 7 days after 2 weeks. Acetamide-45 at concentrations of 5, 10, or 30 mg / kg wasthen administered by intraperitoneal injection. Changes in dynamic lung compli-ance and lung resistance, the accumulation of inflammatory Cells in bronchoalveolar vessel, PDE4 activity, and the concentration of interleukin-4 in rat lung tissue were determined. Results: Seven days of treatment with acetamide-45 preventedeosinophil accumulation in allergic rats. At doses of 5, 10, and 30 mg / kg, aceta -mide-45 decreased lung resistance to 0.20 ± 0.04, 0.25 ± 0.07, and 0.22 ± 0.05 cmH 2 O · s -1 · mL -1, respectively (P <0.05 vs OVA), and it also increased dynamic lung compliance to 0.41 ± 0.07, 0.39 ± 0.06, and 0.42 ± 0.09 mL / cmH20 (P <0.05 vs OVA). After being treated with different doses of acetamide-45, the PDE4 activities in human tissue were 281 ± 55,273 ± 57, and 238 ± 36 nmol · g -1 min -1 (P <0.05 vs OVA), and the concentrations of interleukin-4 in lung tissue were 6.22 ± 1.13, 5.95 ± 1.20, and 5.68 ± 2.20 μtg / g protein (P <0.05 vs OVA) .Conclusions: Acetamide-45 wasfound to improve respiratory function and inhibit airway inflammation in this animal model, and the PDE4 activity of lung tissue was obviously inhibited. Acetamide-45 was an effective anti-inflammatory agent in respiratory inflammation, and the mechanism of its action might depend on inhibition of PDE4.