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作者对103例绒毛标本进行了细胞遗传学分析,检出6例异常,其中3例经胎儿和新生儿组织学证实,另3例未经证实。由此提出继续妊娠中绒毛活检的细胞遗传学可靠性的看法。绒毛取材后使用两利制备方法:一是含滋养叶细胞的完整绒毛中有丝分裂细胞的直接分析法;二是来自绒毛的间叶细胞培养物分析法。在直接法中注意了取材后立即在解剖镜下观察、拍照、估计量,切去非绒毛组织后进行培养。在培养法中应用了胰酶处理法和胰酶-EDTA-分为C、E_2和F等类型。每天监视培养物,直至分裂细胞最多时收获,或根据情况决定是否传代。收获时用0.7%枸橼酸钠低渗20分钟后更换4次甲醇/
The authors conducted a cytogenetic analysis of 103 villi specimens and found 6 abnormalities, of which 3 were confirmed histologically by the fetus and the newborn and the other 3 were unconfirmed. This led to the idea of cytogenetic reliability of villus biopsy in continuing pregnancy. After the use of villus obtained two methods of preparation: First, trophoblastic leaf cells in the complete mitosis of mitotic cells in the direct analysis; the second is from villi mesenchyme culture assay. In the direct method of attention drawn immediately after the dissection microscope observation, photography, estimation, removal of non-hair tissue after culture. Pancreatic enzyme method and trypsin-EDTA-C were used in the culture method and divided into C, E_2 and F types. Cultures are monitored daily until the most dividing cells are harvested or, depending on the situation, the passage is made. Harvest with 0.7% sodium citrate 20 minutes after the infiltration 4 times the replacement of methanol /