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本实验的目的是观察疟疾抗体在体外对多形核白细胞吞噬恶性疟原虫感染的红细胞的影响。实验用的多形核白细胞,系自A型人血经聚蔗糖-泛影葡胺分离提纯得来。恶性疟原虫感染的红细胞,是用体外培养并经离心浓集的方法获得。试验血清分别采用3名冈比亚疟区成人,3名初次患恶性疟并经氯喹治疗的欧洲人和血清库中2份正常人的血清。所有试验血清均经间接荧光技术测定抗体水平。吞噬作用的测定是将感染的或正常的红细胞与多形核白细胞及各试验血清分别混合于试管中,然后在含5%CO_2和95%空气的37℃湿盒中孵育30分钟,再经离心沉淀、涂片,用May-GrunWald-吉氏染色法染色,观察吞噬作用。观察指标为200个多形核白细胞中吞噬红细胞者的百分率,及100个多形
The purpose of this experiment was to investigate the effect of malaria antibodies on phagocytosis of P. falciparum-infected erythrocytes by polymorphonuclear leukocytes in vitro. Experimental polymorphonuclear leukocytes, from type A human blood by Ficoll - meglumine diacetate isolated. Plasmodium falciparum-infected erythrocytes are obtained by culturing in vitro and concentrating by centrifugation. Serum samples were obtained from 3 adults of the Gambia malaria community, 3 from Europeans who first developed falciparum malaria and who were treated with chloroquine, and 2 normal individuals from the serum pool, respectively. All test sera were measured by indirect fluorescent antibody levels. Phagocytosis was assayed by mixing infected or normal erythrocytes with polymorphonuclear leukocytes and each test serum in tubes, followed by incubation for 30 minutes in a 37 ° C wet box containing 5% CO 2 and 95% air, followed by centrifugation Precipitation, smear, staining with May-GrunWald-Giemsa staining to observe phagocytosis. Observed index of 200 polymorphonuclear leukocytes phagocytosed erythrocyte percentage, and 100 polymorphism