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以水稻品种‘空育131’为材料,进行2、4、6、8 d没顶淹水处理并恢复3 d,研究不同程度淹水对水稻生长及抗氧化酶活性的影响。结果表明,随淹水处理时间的延长,叶片和节间显著伸长,导致水稻徒长;光合色素及相对含水量下降;过氧化氢(H2O2)含量逐渐升高,丙二醛(MDA)含量先升后降,在没顶淹水4 d时达到顶点;超氧化物歧化酶(SOD)和过氧化物酶(POD)活性先升后降,在没顶淹水6 d时分别增加80.91%和51.54%,过氧化氢酶(CAT)活性在没顶淹水8 d比对照增加66.59%,抗坏血酸过氧化物酶(APX)活性相对稳定,谷胱甘肽过氧化物酶(GPX)活性先升后降,在淹水4 d时增加78.82%。去水恢复后水稻幼苗仍倒伏,所有处理的地上和地下部干重均增加,淹水2和4 d后恢复的水稻根冠比上升,淹水6和8 d后恢复的水稻根冠比下降;MDA和H2O2含量持续增加,促使CAT和APX活性相应增加,以参与活性氧的清除。结果表明,淹水导致光合色素含量和植株相对含水量下降,酶促和非酶活性氧防卫系统急剧开启,根冠比失调、植株营养失衡,全株徒长和去水倒伏;恢复期通过提高CAT和APX活性,清除不断增加的活性氧,淹水处理的程度不同,根冠比变化不同,恢复机制也不同。
The rice variety ’Kongyu131’ was used as material for 2, 4, 6 and 8 days without flooding treatment and for 3 days. The effects of flooding on rice growth and antioxidant enzyme activities were studied. The results showed that with prolonged flooding treatment, the leaves and internodes were significantly elongated, leading to lean growth of rice; photosynthetic pigment and relative water content decreased; the content of hydrogen peroxide (H2O2) gradually increased; the content of malondialdehyde And then reached the peak when the flooding was for 4 days. The activities of superoxide dismutase (SOD) and peroxidase (POD) first and then decreased, and increased by 80.91% and 51.54% respectively. Catalase (CAT) activity increased by 66.59% compared with the control, but the activity of ascorbate peroxidase (APX) was relatively stable and the activity of glutathione peroxidase (GPX) After dropping, it increased by 78.82% when it flooded for 4 days. The rice seedlings were still lodging after water dewatering, and the dry weight of above-ground and underground parts of all treatments were increased. The root-shoot ratio of rice restored after 2 and 4 d flooding increased, and the root-shoot ratio of rice restored after 6 and 8 d flooding decreased ; MDA and H2O2 content continued to increase, prompted a corresponding increase in CAT and APX activity, in order to participate in the removal of reactive oxygen species. The results showed that the flooding led to the decrease of photosynthetic pigment content and plant relative water content, the rapid and rapid opening of enzymatic and non-enzymatic active oxygen defense systems, imbalance of root and shoot, imbalance of plant nutrition, And APX activity, the removal of increasing reactive oxygen species, the extent of flooding, root and shoot changes than different, recovery mechanism is also different.