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目的:研究前列泌尿栓对急性前列腺炎的抗菌和抗炎作用。方法:60只小鼠随机分为6组:空白组,模型组,阳性药野菊花栓组,前列泌尿栓高、中、低剂量组,前列泌尿栓组按照0.25,0.19,0.13 g·kg-1剂量直肠给药,3 d后ip临床分离变形杆菌菌液或临床分离大肠埃希菌菌液造成体内细菌感染模型,感染24 h后,再连续给药5 d,观察2周,观察前列泌尿栓对小鼠死亡的保护作用;取前列泌尿栓溶液与血清肉汤培养基混合,制成不同药物质量浓度(250.8,188.1,125.4,62.7,31.4g·L-1)的平皿,每个浓度做4个平皿,加入稀释好的菌液,37℃培养48 h,观察最小杀菌浓度(MBC)和最小抑菌浓度(MIC),观察前列泌尿栓对急性前列腺炎主要致病菌的抑菌和杀菌作用;通过大鼠感染大肠埃希菌或变形杆菌造成急性前列腺炎模型的作用,观察前列泌尿栓的消炎作用。结果:感染变形杆菌或大肠埃希菌的小鼠在给予前列泌尿栓后3个剂量组的死亡率均低于模型组(P<0.05);对金黄色葡萄球菌、沙门菌、铜绿假单胞菌、大肠埃希菌、变形杆菌(临床分离)、大肠埃希菌(临床分离)的MBC分别为62.7,125.4,250.8,188.1,250.8,188.1 g·L-1,MIC分别为31.4,62.7,188.1,125.4,188.1,125.4 g·L-1;对变形杆菌或大肠埃希菌造成的急性前列腺炎模型在给予前列泌尿栓后前列腺脏器系数均明显小于模型组,结果有统计学意义(P<0.05),病理切片显示3个剂量组组织恢复均好于模型组,明显降低大鼠前列腺组织炎性细胞浸润(P<0.05)。结论:前列泌尿栓对急性前列腺炎有良好的抗菌和消炎作用。
Objective: To study the antibacterial and anti-inflammatory effects of anterolateral suppository on acute prostatitis. Methods: Sixty mice were randomly divided into six groups: blank group, model group, positive drug chrysanthemum plug group, high-, middle-, and low-dose urinary thrombus group, and 0.25, 0.19, 0.13 g·kg-. 1 dose of rectal administration, 3 days later, ip clinical isolation of proteobacterial bacteria or clinical isolation of Escherichia coli liquid bacteria caused by bacterial infection in vivo, 24 hours after infection, and then continued for 5 days, observed for 2 weeks, observe the forefront of urinary The protective effect of thrombosis on the death of mice; The urinary suppository solution was mixed with serum broth to make plates with different drug concentration (250.8, 188.1, 125.4, 62.7, 31.4 g·L-1), each concentration. Make 4 dishes, add the diluted broth, and incubate for 48 h at 37 °C. Observe the minimum bactericidal concentration (MBC) and the minimum inhibitory concentration (MIC). Observe the bacteriostatic action of the former urinary tract on the major pathogens of acute prostatitis. Bactericidal effect; Through the infection of Escherichia coli or Proteus bacteria in rats caused by the role of acute prostatitis model, observe the anti-inflammatory effect of the forefront urinary thrombi. RESULTS: The mortality of mice infected with Proteus or E. coli in the three dose groups after the administration of urinary suppository was lower than that in the model group (P<0.05); Staphylococcus aureus, Salmonella, and Pseudomonas aeruginosa The MBCs of bacteria, Escherichia coli, Proteus (clinical separation), and Escherichia coli (clinical separation) were 62.7, 125.4, 250.8, 188.1, 250.8, 188.1 g·L-1, respectively, and the MICs were 31.4, 62.7, respectively. 188.1,125.4,188.1,125.4 g·L-1; Prostatitis model of prostatitis caused by Proteus or Escherichia coli was significantly lower than that of model group after administration of urinary suppository, and the results were statistically significant (P <0.05), pathological sections showed that the tissue recovery of the three dose groups was better than that of the model group, and the inflammatory cell infiltration in the prostate tissue was significantly reduced (P<0.05). Conclusion: Forefront urinary thrombi has good antibacterial and anti-inflammatory effects on acute prostatitis.