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目的:观察益气养阴、活血化瘀、清热解毒中药复方“益胃冲剂”对大鼠胃黏膜内源性PGE2及上皮细胞内粘蛋白的影响.方法:实验采用Indomethacin为胃黏膜损伤剂,米索前列醇(PGE1)为对照药物,实验动物为Ⅱ级♂SD大鼠;实验动物共分为两批,两批实验条件一致;每批完全随机化分为四组(n=32):控制组(S):0.9NaCl%+0.9NaCl%、损伤组(M):0.9NaCl%+吲哚美辛、益胃冲剂治疗组(YM):益胃冲剂+吲哚美辛、米索前列醇治疗组(PM):米索前列醇+吲哚美辛;所有大鼠24h禁食不禁水后,每组先后相隔30min两次经口灌胃给药;最后一次给药4h后,腹腔麻醉下手术摘除胃.第一批大鼠用于刮取胃黏膜,放射免疫检测胃黏膜内源性PGE2含量(ng/kg);第二批大鼠用于制作病理切片,在PAS—AB染色和PAS—AB—苏木精染色病理切片上,放大400倍,进行计算机图像定量分析胃黏膜表面上皮细胞内粘蛋白和黏膜内黏液指数(%).结果:胃黏膜内源性PGE2含量:益胃冲剂和米索前列醇均可保持PGE2含量明显高于Indomethacin损伤组(200.0±61.1pg/g&302.9±77.2pg/gvs35.6±16.5pg/g,P<0.01),但也明显低于控制组(vs636.9±104.9pg/g,P<0.01)的水平,胃窦部的结果与胃体部相似.在胃体部两治疗组组间比较有明显的差别(P<0.01),而在胃窦部却无差别;胃黏膜上皮细胞内粘蛋白指数:益胃冲剂可使粘蛋白保持在控制组和损伤组水平以上(41.1±3.3vs35.1±4.9&17.9±3.8,P<0.01),米索前列醇与益胃冲剂及控制组比较无差别(38.2±4.0vs41.1±3.3&35.1±4.9,P>0.05);胃黏膜内黏液指数:益胃冲剂可使黏液指数保持在与控制组及米索前列醇治疗组相当的水平(10.2±3.3vs11.7±3.0&7.8±2.1,P>0.05),上述三组与损伤组比较均有明显差异(vs3.8±1.3,P<0.01).结论:益胃冲剂对胃窦部较胃体部黏膜细胞有更好的促进PGE2合成作用,其效力相当于外源性给予米索前列醇的黏膜保护效力,而对胃体部黏膜细胞PGE2合成的促进作用却低于外源性给予米索前列醇的黏膜保护效力;但益胃冲剂能明显增加胃体部黏膜表面上皮细胞内粘蛋白含量和黏膜内黏液量,与米索前列醇作用相当,而这种促进黏液合成的作用与促进胃黏膜内源性前列腺素素E2的合成有关,同时也明显提示与其他胃黏膜保护机制有关.
Objective: To observe the effects of Yiqi Yangyin, promoting blood circulation, clearing away heat and toxin decoction on the endogenous PGE2 and epithelial mucin in rat gastric mucosa. Methods: Indomethacin was used as gastric mucosal damage agent in the experiment. Misoprostol (PGE1) was used as a control drug and the experimental animals were grade II ♂SD rats; the experimental animals were divided into two batches, and the experimental conditions of the two batches were the same; each batch was completely randomized and divided into four groups (n=32): Control group (S): 0.9 NaCl% + 0.9 NaCl%, Injury group (M): 0.9 NaCl% + indomethacin, Yiwei granules treatment group (YM): Yiwei granules + indomethacin, miso Alcohol treatment group (PM): misoprostol + indomethacin; after 24 hours of fasting and water in all rats, each group was administered by oral gavage at intervals of 30 minutes; intraperitoneal anesthesia was given 4 hours after the last administration. The stomach was removed by surgery. The first group of rats was used to scrape the gastric mucosa, radioimmunoassay was used to measure the endogenous PGE2 content in the gastric mucosa (ng/kg), and the second group of rats was used to make pathological sections and stained with PAS-AB. On PAS-AB-hematoxylin staining pathological sections, the magnification was 400-fold, and computer images were used to quantify the mucin and mucous intramucosal mucus index (%) in the epithelial cells of the gastric mucosa. : Endogenous PGE2 content in gastric mucosa: Yiwei granules and misoprostol can maintain PGE2 content significantly higher than Indomethacin injury group (200.0±61.1pg/g&302.9±77.2pg/gvs35.6±16.5pg/g, P<0.01), but also significantly lower than the control group (vs 636.9±104.9 pg/g, P<0.01). The gastric antrum results are similar to that of the stomach body. There was a significant difference (P<0.01), but there was no difference in the gastric antrum; the intracellular mucin index in the gastric mucosal epithelial cells: Yiwei Granules kept the mucins above the level of the control and injury groups (41.1±3.3 vs. 35. 1 ± 4.9 & 17.9 ± 3.8, P <0.01), there was no difference between misoprostol and Yiwei Granules and control group (38.2 ± 4.0 vs. 41.1 ± 3.3 & 35.1 ± 4.9, P> 0.05); gastric mucosa Internal mucus index: Yiwei granules can keep the mucus index at the same level as the control group and misoprostol treatment group (10.2±3.3vs11.7±3.0&7.8±2.1, P>0.05). There were significant differences in the injury group (vs3.8±1.3, P<0.01). Conclusion: Yiwei Granules have better effect on the synthesis of PGE2 than gastric mucosal cells in gastric antrum, its efficacy is equivalent to exogenous Given the protective efficacy of misoprostol, The promoting effect of PGE2 synthesis in gastric mucosal cells was lower than that of exogenously administered misoprostol; however, Yiwei granules can significantly increase the mucin content in the epithelial cells and mucous volume in the mucous membrane of the gastric mucosa. It is equivalent to misoprostol, and this role in promoting mucus synthesis is related to promoting the synthesis of endogenous prostaglandin E2 in gastric mucosa, and it is also obviously related to other gastric mucosal protective mechanisms.