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目的：确定离体灌注状态下，兔眼虹膜睫状体的突触前５－ＨＴ受体对３Ｈ－乙酰胆硷释放的调节作用．方法：给体外灌注培养虹膜睫状体加入３Ｈ－胆硷，电刺激四次（Ｓ１，Ｓ２，Ｓ３，Ｓ４），刺激强度为３Ｈｚ，５０ｍＡ，１ｍｉｎ．在Ｓ２，Ｓ３，Ｓ４前加入试剂．电刺激诱导的３Ｈ－乙酰胆硷释放的水平通过计算（Ｓｘ／Ｓ１）刺激比率来确定．离子交换色谱法分离灌注液样品中３Ｈ－乙酰胆硷．液内计数仪测定标本中３Ｈ－乙酰胆硷的含量．结果：５－ＨＴ（１ｎｍｏｌ／Ｌ～１０ｍｏｌ／Ｌ）诱导的虹膜睫状体３Ｈ－乙酰胆硷释放的剂量反应曲线呈浓度依赖性（ＥＣ５０＝５８ｎｍｏｌ／Ｌ）．浓度为１ｍｏｌ／Ｌ时，５－ＨＴ诱导的３Ｈ－Ａｃｈ释放量最大，增加了（４５．１４±７．４０）％（ｎ＝６）．给组织灌注５－ＨＴ３拮抗剂Ｔｒｏｐｉｓｅｔｒｏｎ（１ｎｍｏｌ／Ｌ）及５－ＨＴ１拮抗剂Ｍｅｔｈｉｏｔｈｅｐｉｎ（０．１μｍｏｌ／Ｌ），５－ＨＴ（１μｍｏｌ／Ｌ）诱导的３Ｈ－Ａｃｈ释放分别为对照组的８９．９６％及８８．７８％；而加入非选择性５－ＨＴ拮抗剂Ｍｉ－ａｎｓｅｒｉｎ（１０μｍｏｌ／Ｌ）及５－ＨＴ４拮抗剂ＳＤＺ－２０５５５７（０．１μｍｏｌ／Ｌ）时，５－ＨＴ（１μｍｏ?
OBJECTIVE: To determine the regulatory effect of pre-synaptic 5-HT receptors on the release of 3H-acetylcholine in the iris ciliary body of rabbit under perfusion. Methods: The iris ciliary body was infused with 3H-choline and stimulated four times (S1, S2, S3, S4) in vitro. The stimulation intensity was 3Hz, 50mA, 1min. Add reagents before S2, S3, S4. The level of 3H-acetylcholine release induced by electrical stimulation was determined by calculating the ratio of (Sx / S1) stimulation. Separation of 3H-acetylcholine in perfusate samples by ion-exchange chromatography. Determination of 3H-acetylcholine content in samples by liquid counter. Results: The dose-response curve of 3H-acetylcholine release induced by 5-HT (1nmol / L ~ 10mol / L) was concentration-dependent (EC50 = 58nmol / L). The concentration of 1mol / L, 5-HT-induced 3H-Ach release the largest increase (45.14 ± 7.40)% (n = 6). Tissue perfusion with 5-HT3 antagonist Tropisetron (1nmol / L) and 5-HT1 antagonist Methiothepin (0.1μmol / L) and 5-HT (1μmol / L) induced 3H-Ach release were 89 in the control group. The results showed that 5-HT (1μmo? L-96) and 88.78% (88.78%) of the untreated 5-HT antagonist SDZ-205557