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目的:制备黄芩苷纳米结构脂质载体,并考察对大鼠心脏缺血/再灌注损伤的保护作用。方法:采用热熔乳化超声-低温固化法制备黄芩苷纳米结构脂质载体,并考察其包封率、粒径分布、Zeta电位、微观形态及体外释放行为;考察黄芩苷纳米结构脂质载体对大鼠心脏缺血/再灌注损伤的保护作用。结果:黄芩苷纳米结构脂质载体的包封率为(92.1±3.6)%,平均粒径为(241.6±52.2)nm,Zeta电位为(-33.7±2.2)m V,透射电镜显示黄芩苷纳米结构脂质载体成圆整、规则球形。黄芩苷纳米结构脂质载体中药物的体外释放符合Higuchi方程(Q=0.5316t~(1/2)+3.5415,r=0.9590)。黄芩苷纳米结构脂质载体可以增加大鼠心脏缺血/再灌注损伤的保护作用。结论:黄芩苷纳米结构脂质载体对大鼠心脏缺血/再灌注损伤具有良好的保护作用。
OBJECTIVE: To prepare baicalin nanostructured lipid carrier and study its protective effect on myocardial ischemia / reperfusion injury in rats. Methods: The baicalin nanostructured lipid carrier was prepared by hot-melt emulsification ultrasound-low temperature curing method. The encapsulation efficiency, particle size distribution, Zeta potential, microscopic morphology and in vitro release of baicalin were investigated. The effect of baicalin nanostructured lipid carrier pair Protective effects of heart ischemia / reperfusion injury in rats. Results: The entrapment efficiency of baicalin nanostructured lipid carrier was (92.1 ± 3.6)%, the average particle diameter was (241.6 ± 52.2) nm and the zeta potential was (-33.7 ± 2.2) mV. Structural lipid carrier into a round, regular spherical. In vitro release of baicalin nanostructured lipid carrier in line with the Higuchi equation (Q = 0.5316t ~ (1/2) + 3.5415, r = 0.9590). Baicalin nanostructured lipid carrier can increase the protective effect of myocardial ischemia / reperfusion injury in rats. Conclusion: Baicalin nanostructured lipid carrier has a good protective effect on myocardial ischemia / reperfusion injury in rats.