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几种动物的胚泡都合成前列腺素(PG),但其确切功能尚不清楚。本实验欲测定是否前列腺素合成抑制剂消炎痛会抑制滋养层组织对~(22)Na的摄取。动情期的母羊交配16天后杀死,取出胚泡切成长约2mm的切片放于含2ml培养液的培养皿中培养。在37℃通45%O_2、5%CO_2、50%N_2。从6只羊来的滋养属切片分成五组,分别含消炎痛0、0.2、0.4、0.8和1.6mM。取出24和48小时的培养液以放免法测定PGF_(2α)和PGFM的含量,用二苯胺反应测定组织的DNA含量。PGF_(2α)和PGFM以每μgDNA相应含量表示。结果,含0、0.2、0.4、0.8和1.6mM消炎痛组释放的PGF_(2α)分别是205.0±71.2、3.3±0.2、1.9±0.2、0.8±0.1、0.15±0.05ng/μgDNA释放的PGFM分别是0.7±0.1、
Prostaglandins (PGs) are synthesized in the blastocysts of several species of animals, but its exact function is unclear. This experiment was conducted to determine if indomethacin, a prostaglandin synthesis inhibitor, inhibits uptake of ~ (22) Na in trophoblast tissue. Estrus of ewes killed after mating 16 days, remove the blastocyst cut into about 2mm slices containing culture medium containing 2ml dish culture. At 37 ℃ through 45% O_2, 5% CO_2, 50% N_2. Trophobiotics from 6 sheep were divided into five groups containing indomethacin 0, 0.2, 0.4, 0.8 and 1.6 mM, respectively. Twenty-four and 48 hours after the culture medium was removed, the contents of PGF_ (2α) and PGFM were determined by radioimmunoassay, and the DNA content of the tissues was determined by diphenylamine reaction. PGF_ (2α) and PGFM are expressed as the corresponding contents per μg of DNA. As a result, the release of PGF 2α by 0, 0.2, 0.4, 0.8 and 1.6 mM indomethacin groups was 205.0 ± 71.2, 3.3 ± 0.2, 1.9 ± 0.2, 0.8 ± 0.1 and 0.15 ± 0.05 ng / μg, respectively Is 0.7 ± 0.1,