THE COMPLETE NUCLEOTIDE SEQUENCE OF THE CLONED DNA OF HEPATITIS B VIRUS SUBTYPE adr IN pADR-1

• 作 者: 甘人宝 储美瑾 沈绿萍 钱苏雯 李载平
• 机 构: Shanghai Institute of Biochemistry, Academia Sinicarrrrrrrrn,Shanghai Institute of Biochemi
• 出 处: Science in China,Ser.B
• 发表日期: 1987年05期
• 关键词: DNA 江口 巴口 Gilbert cloned heterogeneity 卜山 二山 homogeneity subtype

The complete nucleotide sequence of the cloned hepatitis B virus DNA subtype adr in pADR-1 wasdetermined by Maxam and Gilbert’s method. It is 3215 base pairs in size, which is 27 bp longer thanthe sequence of the adr pHBr330, as reported by Ono et al. The nucleotide difference between pADR-1and adr pHBr330 is about 2% while those between pADR-1 and adw as well as ayw are 9.3% and 9.7%respectively. In this paper, the heterogeneity and homogeneity of the S gene, the C gene and the othercoding regions in pADR-1 and in the other subtypes are compared and discussed.

The complete nucleotide sequence of the cloned hepatitis B virus DNA subtype adr in pADR-1 was determined by Maxam and Gilbert’s method. It is 3215 base pairs in size, which is 27 bp longer thanthe sequence of the adr pHBr330, as reported by Ono et al The nucleotide difference between pADR-1 and adr pHBr330 is about 2% while those between pADR-1 and adw as well as ayw are 9.3% and 9.7% respectively. In this paper, the heterogeneity and homogeneity of the S gene, the C gene and the other coding regions in pADR-1 and in the other subtypes are compared and discussed.