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目的探讨过氧化物酶体增殖物活化受体γ(PPAR-γ)配体罗格列酮对大鼠脓毒症急性肺损伤的保护作用。方法 90只SD大鼠随机均分为假手术组(SO组)、改良盲肠结扎穿孔术手术组(CLP组)和罗格列酮4mg.kg-1.d-1治疗组(RT组)。术后2、6、12、24、48h检测大鼠动脉血氧分压(PaO2)、支气管-肺泡灌洗液(BALF)中白细胞(WBC)计数和丙二醛(MDA)含量,HE染色观察肺组织病理改变。结果与SO组相比,CLP组术后肺泡肺间质水肿和炎症细胞渗出加重,PaO2显著下降,BALF中WBC计数和MDA含量显著升高(P<0.05);而RT组的上述指标均明显改善(P<0.05)。结论 PPAR-γ配体对大鼠脓毒症急性肺损伤有保护作用。
Objective To investigate the protective effect of peroxisome proliferator-activated receptor γ (PPAR-γ) ligand rosiglitazone on acute lung injury induced by sepsis in rats. Methods Ninety Sprague-Dawley rats were randomly divided into sham operation group (SO group), cecal ligation and puncture operation group (CLP group) and rosiglitazone 4 mg.kg-1.d-1 treatment group (RT group). PaO2, WBC and MDA in bronchoalveolar lavage fluid (BALF) at 2, 6, 12, 24, and 48 h after operation were measured. HE staining Lung tissue pathology. Results Compared with SO group, the pulmonary alveolar interstitial edema and inflammatory cell exudation in the CLP group were significantly increased, the PaO2 significantly decreased, and the WBC count and MDA content in the BALF were significantly increased (P <0.05); while in the RT group, Significant improvement (P <0.05). Conclusion PPAR-γ ligand has a protective effect on acute lung injury induced by sepsis in rats.