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目的:观察抑胃多肽(GIP)靶向的主动免疫干预治疗对大鼠行为学的影响。方法:高脂喂养的SD大鼠分为实验组及对照组。实验组如期皮下多点注射GIP靶向的人工抗原(GIP-KLH);对照组如期皮下多点注射KLH。检测血清中GIP抗体滴度水平,观察疫苗诱生抗体对GIP促进胰岛β细胞MIN6细胞分泌胰岛素的影响及两组在行为学方面存在的差异。结果:实验组首次免疫接种后4周,即产生了高滴度GIP特异性抗体,如期继续免疫接种,抗体水平进一步增高;实验组血清较对照组可以显著抑制GIP的促MIN6细胞胰岛素分泌作用(P<0.01);行为学实验中,自发活动测试实验组总路程(P<0.01)、平均速度(P<0.01)、活动时间(P<0.05)、活动次数(P<0.01)均明显低于对照组,休息时间明显高于对照组(P<0.05),有统计学差异,Morris水迷宫测试两组无明显统计学差异(P>0.05)。结论:人工抗原GIP-KLH能打破机体免疫耐受,诱生GIP特异性抗体;GIP靶向的主动免疫干预治疗使大鼠自发活动性下降。
Objective: To observe the effect of GIP-targeted active immune intervention on behavior in rats. Methods: SD rats fed with high fat diet were divided into experimental group and control group. In the experimental group, GIP-targeted artificial antigen (GIP-KLH) was subcutaneously injected subcutaneously. In the control group, KLH was injected subcutaneously on multiple occasions. The level of GIP antibody titers in serum was detected to observe the effect of antibody induced by GIP on insulin secretion induced by GIP in MIN6 cells of pancreatic islet cells and the difference in behavior between the two groups. RESULTS: Four weeks after the first immunization, GIP-specific antibody was produced in high titer group. The immunization continued with continued immunization with antibody levels increasing. The serum of experimental group significantly inhibited the insulin secretion of GIP-induced MIN6 cells P <0.01). In the behavioral experiments, the total distance traveled (P <0.01), average speed (P <0.01), activity time The resting time in the control group was significantly higher than that in the control group (P <0.05). There was no significant difference between the two groups in Morris water maze test (P> 0.05). Conclusion: The artificial antigen GIP-KLH can break the immune tolerance and induce the GIP-specific antibody. The GIP-targeted active immune intervention decreased spontaneous activity in rats.