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Aim:The present study aimed to elucidate the role of T-subtype calcium channels(Ca_v3.1,Ca_v3.2,and Ca_v3.3)in the pathogenesis of neuropathic pain at spinal level.Methods:The chronic compression of the dorsal root ganglion(CCD)rat modelwas adopted.The antisense oligonucleotide of Ca_v3.1,Ca_v3.2,and Ca_v3.3 or nor-mal saline(NS)were intrathecally administered twice per day from the first day tothe fourth day after operation.Paw mechanical withdrawal threshold and pawthermal withdrawal latency were measured to evaluate the tactile allodynia andthermal hyperalgesia,respectively.Results:CCD rats developed reliable tactileallodynia and thermal hyperalgesia after operation.Intrathecal administration ofantisense oligonucleotide of Ca_v3.2 and Ca_v3.3 significantly relieved tactileallodynia and thermal hyperalgesia in CCD rats,but not Ca_v3.1.Conclusion:Ca_v3.2and Ca_v3.3 subtype calcium channels in the spinal cord may play an important rolein the pathogenesis of neuropathic pain,which may contribute to the manage-ment of the neuropathic pain.
Aim: The present study aimed to elucidate the role of T-subtype calcium channels (Ca_v3.1, Ca_v3.2, and Ca_v3.3) in the pathogenesis of neuropathic pain at spinal level. Methods: The chronic compression of the dorsal root ganglion (CCD) rat model was coated. The antisense oligonucleotide of Ca_v3.1, Ca_v3.2, and Ca_v3.3 or nor-mal saline (NS) were intrathecally administered twice per day from the first day tothe fourth day after operation. Paw mechanical withdrawal threshold and pawthermal withdrawal latency were measured to evaluate the tactile allodynia andthermal hyperalgesia, respectively. Results: CCD rats developed reliable tactile allodynia and thermal hyperalgesia after operation. Intrathecal administration ofantisense oligonucleotide of Ca_v3.2 and Ca_v3.3 significantly relieved tactile allodynia and thermal hyperalgesia in CCD rats, but not Ca_v3.1.Conclusion: Ca_v3.2 and Ca_v3.3 subtype calcium channels in the spinal cord may play an important role in the pathogenesis of neuropathic pain, which may c ontribute to the manage-ment of the neuropathic pain.