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目的建立五色梅中β-榄香烯的含量分析方法,并对四川攀西地区恶性杂草植物五色梅中的β-榄香烯含量和分布规律进行测定分析。方法采用HPLC测定五色梅样品中β-榄香烯含量,Eclipse XDB-C18色谱柱(4.6 mm×150 mm,5μm),流动相:乙醇-乙腈-水=70︰10︰20,流速:1.0 mL.min-1,检测波长:210 nm,进样量:20μL,柱温:30℃。用TLC和紫外光谱对样品中的β-榄香烯进行鉴定分析。结果 HPLC测β-榄香烯含量在0.007 5～0.120 0 mg.mL-1内线性关系良好(r=0.999 5),平均加样回收率为99.3%。五色梅叶和花中均含β-榄香烯,而以叶中含量最高,鲜叶与花中含量分别为0.025%和0.007%。鲜叶和自然晒干叶中β-榄香烯含量差异无统计学意义,但均显著高于60℃烘干叶。结论本法适用于五色梅中β-榄香烯的含量测定,本研究可为开辟新的β-榄香烯来源、五色梅的开发利用和治理提供参考。
OBJECTIVE To establish a method for the determination of β-elemene in Plum color, and to determine the content and distribution of β-elemene in the weedy plant Plum color in Panxi, Sichuan Province. Methods The content of β-elemene was determined by HPLC on a Eclipse XDB-C18 column (4.6 mm × 150 mm, 5 μm). The mobile phase was ethanol-acetonitrile-water = 70︰10︰20 and the flow rate was 1.0 mL .min-1, detection wavelength: 210 nm, injection volume: 20μL, column temperature: 30 ℃. Identification of β-elemene in samples by TLC and UV spectroscopy. Results The linearity of β-elemene was 0.007 5 ~ 0.120 0 mg.mL-1 (r = 0.999 5). The average recovery was 99.3%. Elemental and elemene contained P-elemene, while the content was the highest in leaves, the contents of fresh leaves and flowers were 0.025% and 0.007% respectively. The content of β-elemene in fresh leaves and natural leaves was not significantly different, but both were significantly higher than those in dried leaves at 60 ℃. Conclusion This method is suitable for the determination of β-elemene in Plum color. This study may provide a reference for the development of a new source of β-elemene, development and utilization of colored Plum.