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为探索球形红细菌对丹参的生物转化机理,采用非变性聚丙烯酰胺凝胶电泳(N-PAGE)分别对丹参水提液、醇提液、醇水提液培养后球形红细菌菌体及纯球形红细菌(PRS)菌体蛋白质(Pro)、酯酶(EST)、细胞色素氧化酶(COD)、超氧化物歧化酶(SOD)和过氧化物酶(POD)进行分析,比较用丹参提取液培养前后球形红细菌菌体蛋白及4种酶的同工酶变化.结果表明:丹参提取液培养前后球形红细菌菌体蛋白及4种酶的同工酶谱带差别较大,同工酶的电泳迁移率、活性、所表达同工酶的数目及分布均有差异,培养d 2～6蛋白及酶表达量变化最大,d 14～20基本稳定.研究表明丹参能诱导球形红细菌生成新的蛋白质及酶,亦可抑制某些蛋白质和酶的合成;这些蛋白和酶可能参与了丹参化学成分的生物转化.图4参22
In order to explore the bioconversion mechanism of Rhodobacter sphaeroides to Salvia miltiorrhiza, non-denaturing polyacrylamide gel electrophoresis (N-PAGE) was used to detect the growth of Rhodobacter sphaeroides and pure Rhizoctonia solani Escherichia coli, Rhizopus sp., Escherichia coli, Rhizopus sp., Escherichia coli, ProS, EST, COD, SOD and POD of Rhodobacter sphaeroides were compared. The results showed that isozyme bands of Rhodobacter sphaeroides and four kinds of enzymes were significantly different before and after Salvia miltiorrhiza extract culture, isoenzyme The results showed that Salvia miltiorrhiza could induce the formation of Rhodobacter sphaeroides. The results showed that Salvia miltiorrhiza could induce the formation of new Rhodobacter sphaeroides Of proteins and enzymes, but also inhibit the synthesis of certain proteins and enzymes; these proteins and enzymes may be involved in the biological transformation of Salvia miltiorrhiza composition.