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目的:建立大鼠慢性心理性应激胃溃疡模型,观察加味四逆散(JWSNS)对模型大鼠胃黏膜形态、血浆促肾上腺皮质激素(ACTH)、血清皮质醇(CORT)和黏膜血流量等的影响,探讨其保护胃黏膜的机制。方法:Wistar大鼠随机分为正常组,模型组,加味四逆散高、中、低剂量(10,5,2.5 g.kg-1)组,奥美拉唑(3.0×10-3g.kg-1)组,除正常组外,其余各组采用10种不可预知刺激法建立慢性大鼠心理性应激胃溃疡模型,灌胃给药4周后,常规HE染色观察胃组织形态学改变及胃黏膜溃疡指数(UI),多道生理记录仪检测胃黏膜血流量(GMBF),放免γ计数器检测血浆ACTH,血清CORT的变化。结果:模型组大鼠胃黏膜弥漫出血、溃疡糜烂,其胃黏膜UI,血浆ACTH,血清CORT与正常组比较均明显升高(P<0.01),GMBF,胃黏膜修复有效率与正常组比较均明显下降(P<0.01),加味四逆散高、中、低剂量组大鼠胃黏膜损伤较模型组不同程度减轻,其胃黏膜UI,血浆ACTH,血清CORT与模型组比较均明显下降(P<0.01或P<0.05),而GMBF,胃黏膜修复有效率与模型组比较均明显升高(P<0.01或P<0.05)。结论:慢性心理性应激对大鼠胃黏膜有明显损伤作用,加味四逆散能显著减轻心理性应激导致的胃黏膜损伤,有效减低溃疡指数,调整神经内分泌功能,增加GMBF和提高胃黏膜修复能力。
OBJECTIVE: To establish a rat model of gastric ulcer induced by chronic psychological stress and to observe the effects of JWSNS on gastric mucosal morphology, plasma adrenocorticotropic hormone (ACTH), serum cortisol (CORT) and mucosal blood flow To explore its mechanism of protecting gastric mucosa. Methods: Wistar rats were randomly divided into normal group, model group, Jiawei Sini San high, medium and low dose (10,5,2.5 g.kg-1) group, omeprazole (3.0 × 10-3g.kg -1) group. In addition to the normal group, the rest of the groups using 10 kinds of unpredictable stimulation to establish chronic psychological stress rat model of gastric ulcer, 4 weeks after oral administration, conventional HE staining observed gastric tissue morphology changes and Gastric mucosal ulcer index (UI), gastric mucosal blood flow (GMBF) by multi-channel physiology recorder and ACTH and serum CORT by radioimmunoassay. Results: Gastric mucosa was diffusely hemorrhaged and gastric ulcer was eroded in model group. The gastric mucosal UI, plasma ACTH and serum CORT were significantly increased compared with normal group (P <0.01), and the effective rate of GMBF and gastric mucosal repair were (P <0.01). Gastric mucosal lesion in rats treated with Jiawei Sini San high, medium and low dose groups was lessened to a certain extent compared with model group. The gastric mucosal UI, plasma ACTH and serum CORT were significantly decreased compared with model group (P <0.01 or P <0.05). However, the effective rate of GMBF and gastric mucosa repair was significantly higher than that of model group (P <0.01 or P <0.05). Conclusion: Chronic psychological stress can significantly damage gastric mucosa in rats. Jiawei Sini Decoction can significantly reduce the gastric mucosal injury caused by psychological stress, effectively reduce the ulcer index, regulate neuroendocrine function, increase GMBF and improve gastric mucosa Repair ability.