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The source of systematic frequency shift of single-ion optical frequency standard of 40Ca+ ion trapped in endcap trap and laser-cooled to a few of mK is analysed and calculated.Second-order Doppler shift,Stark shift due to micromotion and thermal motion.blackbody Stark shift and ac Stark shift are calculated for the ground state and excited 3d 2D5/2 state.The Hertz-level measurement of the optical clock frequeney in a single 40Ca+ ion can be performed.