,A head-to-toe makeover for classical sequencing-by-synthesis helps users to squeeze more out of eac

• 作 者: Jianbin Wang Angela Wu
• 机 构: School of Life Sciences, Tsinghua University,China“,”Division of Life Science, Hong Kong University
• 出 处: 国家科学评论（英文版）
• 发表日期: 2019年1期

Today,many next-generation sequencing (NGS) methods,technologies and platforms have been developed that allow users to produce Tera base pairs of data in less than one day or ultralong reads covering multiple genes.By far the most popular NGS approach to date is cyclic sequencing-by-synthesis (SBS),whereby each DNA templates complementary strand is synthesized by DNA polymerase and,as the new strand grows base by base,the identity of each incorporated dNTP is detected via different chemistries or modalities.Existing variations on this SBS approach each has its strengths,but also each comes with trade-offs:modalities with high accuracy often suffer from reduced sequencing speed or read length;others can achieve long read length but at the cost of accuracy.In their recent Nature Biotechnology publication [1],Chen and colleagues give the classical SBS method an innovative head-to-toe makeover that could mean yet another quantum leap in sequencing speed,accuracy,and cost reduction.