论文部分内容阅读
目的：探讨肿瘤细胞诱导分化剂六亚甲基二乙酰胺（ＨＭＢＡ）和Ｎ－乙酰－１，６－二胺基己烷（ＮＡＤＡＨ）与人红白血病Ｋ５６２细胞ＤＮＡ的相互作用和诱导肿瘤细胞分化的机理．方法：通过两步合成，将ＮＡＤＡＨ偶联到对甲基苯磺酰氯活化的异丙基甘油硅胶上，制备高效液相色谱柱，研究人红白血病Ｋ５６２细胞ＤＮＡ在ＮＡＤＡＨ柱上的行为及ＮＡＤＡＨ及ＨＭＢＡ与人红白血病Ｋ５６２细胞ＤＮＡ的相互作用．结果：以２ｍｏｌ／Ｌ（ＮＨ４）２ＳＯ４ｐＨ７．４的磷酸缓冲液做降盐梯度洗脱时，Ｋ５６２细胞ＤＮＡ大部分很快被洗脱流出，另有一小部分ＤＮＡ在（ＮＨ４）２ＳＯ４浓度降至最低时才流出，ＮＡＤＡＨ柱对这部分ＤＮＡ的保留能力极强，即有很强的疏水作用；不同的ＤＮＡ与ＮＡＤＡＨ具有不同的疏水亲和力．ＮＡＤＡＨ柱对ＤＮＡ的离子效应很弱．结论：ＨＭＢＡ和ＮＡＤＡＨ在诱导肿瘤细胞的分化过程还对ＤＮＡ有疏水作用．
OBJECTIVE: To investigate the interaction between tumor cells inducing differentiation of HMBA and NADAH in DNA of human erythroleukemia K562 cells and induce tumor cell differentiation The mechanism. METHODS: NADAH was coupled to p-methylbenzenesulfonyl chloride-activated isopropyl glycerol silica gel in two steps to prepare high performance liquid chromatography (HPLC) column. The DNA of human erythroleukemia K562 cells on NADAH column and the effect of NADAH and Interaction between DNA of HMBA and human erythroleukemia K562 cells. Results: Most of the DNA of K562 cells was eluted quickly when desalting with 2 mol / L (NH4) 2SO4 pH7.4 phosphate buffer, and a small part of DNA was minimized at the concentration of (NH4) 2SO4 When the outflow, NADAH column retention of this part of the DNA strong, that is, has a strong hydrophobic effect; different DNA and NADAH have different hydrophobic affinity. The NADAH column has a weak ionic effect on DNA. Conclusion: HMBA and NADAH also induce hydrophobic interaction with DNA in tumor cell differentiation.