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目的：研究维拉帕米对柯萨奇Ｂ３病毒（ＣＶＢ３）感染新生大鼠培养心肌细胞离体模型的影响。方法：制备ＣＶＢ３感染培养的新生ＳＤ（Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ）大鼠搏动心肌细胞。观察细胞病变，在维拉帕米实验组及生理盐水对照组检测培养液中心肌型肌酸激酶同功酶，培养液及细胞悬液中ＣＶＢ３－核糖核酸（ＣＶＢ３－ＲＮＡ），另设实验研究培养心肌细胞钙离子流入量。结果：ＣＶＢ３感染经维拉帕米处理组（５×１０－７及５×１０－８ｍｏｌ／Ｌ，ｎ＝８）细胞病变较生理盐水处理组（ｎ＝８）、地塞米松处理组（１及２×１０－４ｍｏｌ／Ｌ，ｎ＝８）及人体重组干扰素α１组（１０２及１０３Ｕ／Ｌ，ｎ＝８）明显严重（Ｐ均＜０．０１）；生理盐水处理组心肌型肌酸激酶同功酶显著低于维拉帕米处理组（Ｐ＜０．０１）。生理盐水处理组细胞悬液中总核糖核酸与ＣＥＶ２－互补脱氧核糖核酸探针杂交带小于维拉帕米处理组；生理盐水处理组培养液中总核糖核酸与ＣＥＶ２－互补核糖核酸杂交带小于维拉帕米处理组，杂交指数小于维拉帕米处理组（Ｐ＜０．０５）。ＣＶＢ３感染使培养心肌细胞的快速及慢速时相钙内流明显增加（（Ｐ＜０．０５），维拉帕米可减低慢速时相钙内流（Ｐ＜０．０１），但对快速?
OBJECTIVE: To study the effect of verapamil on cultured rat cardiac myocytes isolated from Coxsackie virus B3 (CVB3) infected neonatal rats. Methods: CVB3 infected cultured neonatal SD (Sprague-Dawley) rat heartbeat cardiomyocytes. The changes of CVB3-RNA in CVB3-RNA were detected in the verapamil and saline control groups, respectively Cultivate cardiomyocyte calcium influx. Results: The cytopathic effect of CVB3 infection in verapamil treated group (5 × 10-7 and 5 × 10-8mol / L, n = 8) was significantly lower than that in saline treated group (n = 8) and dexamethasone treated group And 2 × 10-4mol / L, n = 8) and human recombinant interferon α1 group (102 and 103U / L, n = 8) were significantly higher than those in the normal saline group The kinase isozyme was significantly lower than that of verapamil (P <0.01). The hybridization band of total RNA and CEV2-complementary deoxyribonucleic acid probe in the cell suspension of the saline treatment group was smaller than that of the verapamil treatment group; the hybridization band of the total RNA and the CEV2-complementary RNA in the culture medium of the saline group was smaller than that of the CEV2- Laparib treatment group, hybridization index was less than verapamil treatment group (P <0.05). CVB3 infection significantly increased calcium influx in both fast and slow phase of cultured cardiomyocytes (P <0.05), while verapamil decreased slow phase calcium influx (P <0.01) fast?