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试验比较了西瓜枯萎病(Fusarium oxysporum f.sp.niveum)苗期鉴定的4种接种方法和浸根法的不同浓度1×10~5、1×10~4、1×10~5、1×106、1×107孢子/ml的效果.以及杂交一代材料“85—26”的室内鉴定结果与疫区表现。结果表明:胚根法易出现于叶畸形(25.8%～47.2%)和烂籽(53.4%);灌根法潜育期长(16～18天);以浸根法最好,孢子培育法次之,潜育期一周,重复性好,结果稳定。浸根法最适浓度为1×10~5～1×10~6孢子/ml。“85—26”的疫区表现与室内鉴定相符。
The four inoculation methods identified by Fusarium oxysporum f.sp.niveum and the different concentrations of 1 × 10 ~ 5, 1 × 10 ~ 4, 1 × 10 ~ 5, 1 × 106,1 × 107 spores / ml, as well as the cross-generation material “85-26” indoor identification results and epidemic area performance. The results showed that the radicle emergence (25.8% -47.2%) and rotten seeds (53.4%) were easy to occur in radicle explants. The latent period of irrigation was longer (16-18 days) Second, the latent period of one week, good repeatability, the result is stable. The best rooting concentration is 1 × 10 ~ 5 ~ 1 × 10 ~ 6 spores / ml. The epidemic performance of “85-26” is in line with indoor identification.