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为评价生物素-亲和素预定位技术在炎症显像中的应用价值,采用链霉亲和素(SA)和~(113m)In-DTPA-生物素(~(113)In-DB)、生物素-人免疫球蛋白(B-hlgG)和~(131)I-链霉亲和素(~(131)I-SA)行二步法预定位炎症显像,并以~(99m)Tc 直接标记hlgG、SA 一步法显像为对照,于2、6或24小时显像,测定生物分布。实验动物用葡萄球菌所致小鼠炎症模型。结果:SA和~(113m)In-DB 二步法预定位法在2小时即可显像,其靶/非靶(T/NT)比值为1.83,6小时达4.33,血中清除较快;B-hlgG和~(131)I-SA 二步法预定位显像,T/NT 在2小时仅0.39,24小时升至2.70,hlgG 血中清除时间较长,血本底较高。直接标记SA 在肝、肾有较多放射性浓聚;标记 hlgG 分子量较大。其血清除时间长,6小时仍保持较高水平,且肝积聚较多,图像不清晰。结果表明,预定位法的 T/NT 比值较直接标记蛋白质法为高,且显像时间提前;可用于炎症显像,并可提高图像的清晰度。
To evaluate the value of biotin-avidin pre-localization in inflammatory imaging, streptavidin (SA) and ~ (113m) In-DTPA-biotin Inflammatory imaging with biotin-human immunoglobulin (B-hlgG) and 131I-streptavidin (~ (131) I-SA) Direct labeling hlgG, SA one-step imaging as a control, at 2,6 or 24 hours imaging, biodistribution. Experimental animals Staphylococcus aureus-induced mouse inflammation model. Results: SA and ~ (113m) In-DB two-step pretargeting method can be visualized in 2 hours, the target / non-target ratio was 1.83 in 6 hours and 4.33 in serum. B-hlgG and ~ (131) I-SA two-step pre-positioning imaging, T / NT in only 2 hours 0.39,24 hours rose to 2.70, hlgG blood clearance time is longer, the blood background is higher. Direct labeling of SA in the liver, kidney more radioactive accumulation; Marker hlgG larger molecular weight. In addition to a long time of its serum, 6 hours still remained high, and more liver accumulation, the image is not clear. The results showed that the T / NT ratio of the pretargeting method was higher than that of the direct labeling protein method, and the imaging time was advanced. It could be used for the imaging of inflammation and improve the clarity of images.