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目的运用MTT法抗肿瘤生物活性模型对来源于东海海水的10株真菌提取物进行生物活性筛选。方法对10株海水来源真菌的发酵液乙酸乙酯提取物进行体外抗肿瘤活性(MTT法)筛选研究评估。结果菌株12010104、12010103、12010101和08010101的发酵液提取物对PC12肿瘤细胞的细胞毒性较强,当浓度为200μg·mL-1时抑制率可达70%以上。菌株12010104、12010109、及08010101的发酵液提取对HepG2肿瘤细胞物表现出较强的抑制活性,抑制率在浓度为200μg·mL-1时可达85%以上,真菌12010101的抑制活性次之,在同浓度为77%。而菌株12010104、12010101及08010101在浓度为100μg·mL-1时对U937肿瘤细胞具有较强的细胞毒性,抑制率可达90%以上。结论菌株12010104、12010101和08010101的发酵液提取物在体外具有较好的PC12、HepG2、U937细胞毒活性,具有潜在的海洋药物研究价值。
OBJECTIVE To evaluate the bioactivity of 10 fungal extracts from seawater in the East China Sea using MTT anti-tumor bioactivity model. Methods To evaluate the anti-tumor activity (MTT method) screening of 10 ethyl acetate extracts from the fermentation broth of seawater-derived fungi. Results The fermentation broth extracts of strains 12010104, 2010201010, 20102010101 and 08010101 were highly cytotoxic to PC12 tumor cells. The inhibitory rate of PC12 cells reached more than 70% at the concentration of 200μg · mL-1. The strains 12010104,12010109 and 08010101 of the fermentation broth extract showed strong inhibitory activity on HepG2 tumor cells, the inhibitory rate of which reached more than 85% at the concentration of 200μg · mL-1, followed by the fungal strain 12010101, The same concentration of 77%. However, strains 12010104, 20102010101 and 08010101 had strong cytotoxicity on U937 tumor cells with a concentration of 100μg · mL-1, with the inhibition rate of more than 90%. Conclusion The fermentation broth extracts of strains 12010104, 20102010101 and 08010101 have better cytotoxic activity of PC12, HepG2 and U937 in vitro, which has the potential value of marine drug research.