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目的对1株海洋来源的真菌小红酵母Rhodotorula minuta.次级代谢产物进行活性化学成分的分离研究。方法通过抗氧化(DPPH法)和抗肿瘤(MTT法)双模型活性追踪筛选分离菌株发酵液正丁醇萃取部分的活性化学成分,并利用流式细胞仪技术对抗肿瘤机制作初步探究。结果从中得到了1个较高活性环二肽类化合物,利用波谱分析及与文献数据对照等方法鉴定为环亮-异亮氨酸Cyclo(Leu-Ile),为首次从海洋小红酵母菌中分得。在浓度为10μmol.mL-1时,环亮-异亮氨酸对DPPH自由基的清除率率达73.76%;对HepG2、PC-12、U937肿瘤细胞的IC50值分别为0.538,0.635和0.437μmol.mL-1;流式细胞仪检测表明,环二肽能有效诱导U937细胞凋亡。结论该环二肽在体外具有较好的清除DPPH自由基活性,对HepG2、PC12、U937肿瘤细胞具有较好的细胞毒活性,同时也表明海洋真菌是活性天然产物的重要来源。
OBJECTIVE To separate the active metabolites of Rhodotorula minuta, a secondary metabolite of marine origin from Rhodotorula minuta. Methods The chemical constituents of the n-butanol extraction of the fermentation broth of the isolates were screened by DPPH and anti-tumor (MTT) bi-modal activity traces. The anti-tumor mechanism was investigated by flow cytometry. The results obtained from a higher activity of cyclic dipeptide compounds, the use of spectral analysis and comparison with the literature data identified as bright - isoleucine Cyclo (Leu-Ile), for the first time from the marine red yeast Share. At the concentration of 10 μmol · mL-1, the clearance rate of DPPH radical was 73.76%, and the IC50 values of HepG2, PC-12 and U937 cells were 0.538, 0.635 and 0.437 μmol .mL-1; flow cytometry showed that cyclic dipeptide can effectively induce apoptosis of U937 cells. Conclusion The cyclic dipeptide has good DPPH radical scavenging activity in vitro and good cytotoxic activity on HepG2, PC12 and U937 tumor cells. It also shows that marine fungi are important sources of active natural products.