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目的:制备灯盏花素阳离子脂质体和普通脂质体并对其相关性质进行考察。方法:采用薄膜分散法制备灯盏花素脂质体;以包封率为指标,通过正交试验对处方进行优化。考察灯盏花素普通脂质体和阳离子脂质体的体外释放行为。结果:制备的灯盏花素阳离子脂质体性质稳定,包封率为(76.42±1.973)%,平均粒径为(186±35)nm,Zeta电位为(48.9±9.83)mV。灯盏花素普通脂质体和阳离子脂质体的释放过程的拟合方程分别为lnln[1/(1-Q)]=0.7797lnt-2.3187(r=0.9739)和lnln[1/(1-Q)]=0.3553lnt-3.197(r=0.9899)。结论:确定了最优处方,制备得到包封率较高且状态稳定的灯盏花素阳离子脂质体。
OBJECTIVE: To prepare breviscapine cationic liposomes and liposomes and investigate the related properties. Methods: Breviscapine liposomes were prepared by membrane dispersion method. The encapsulation efficiency was used as an index to optimize the prescription by orthogonal test. The in vitro release behavior of breviscapine common liposomes and cationic liposomes was investigated. Results: The prepared breviscapine cationic liposomes were stable in nature with entrapment efficiency of (76.42 ± 1.973)%, average particle diameter of (186 ± 35) nm and zeta potential of (48.9 ± 9.83) mV. The fitting equations for the release process of breviscapine common liposomes and cationic liposomes were lnln [1 / (1-Q)] = 0.7797lnt-2.3187 (r = 0.9739) and lnln [1 / (1-Q )] = 0.3553lnt-3.197 (r = 0.9899). Conclusion: The optimal formulation was determined, Breviscapine cationic liposomes with high encapsulation efficiency and stable state were prepared.