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目的:从定性定量角度探讨蜂毒素诱导骨肉瘤U2OS细胞凋亡的作用。方法:采用MTT法检测蜂毒素对骨肉瘤U2OS细胞的增殖抑制作用,通过激光共聚焦显微镜、Annexin V/PI双标记法及原位缺口末端标记(TUNEL)等检测方法对细胞凋亡作定性定量分析。结果:蜂毒素能显著抑制U2OS细胞的增殖;激光共聚焦显微镜下可见到明显的凋亡细胞;蜂毒素浓度为2μg/ml、4μg/ml、8μg/ml时,应用流式细胞仪检测到U2OS细胞凋亡率分别为5.0211±0.3135、8.1111±1.0208和10.8933±1.7411,与对照组(1.7489±0.9281)相比,差异具有统计学意义(P<0.01),TUNEL结果显示蜂毒素处理组的细胞凋亡指数较对照组显著升高(P<0.01)。结论:蜂毒素能够抑制U2OS细胞增殖并能诱导其凋亡。
Objective: To investigate the effect of melittin in inducing apoptosis of U2OS cells in osteosarcoma from qualitative and quantitative point of view. Methods: The inhibitory effect of melittin on the proliferation of osteosarcoma U2OS cells was detected by MTT assay. The apoptosis of cells was qualitatively and quantitatively determined by laser confocal microscopy, Annexin V / PI double staining and TUNEL. analysis. Results: The melittin could significantly inhibit the proliferation of U2OS cells. The apoptotic cells were observed under laser scanning confocal microscope. When the concentration of melittin was 2μg / ml, 4μg / ml and 8μg / ml, the expression of U2OS was detected by flow cytometry The apoptotic rates were 5.0211 ± 0.3135, 8.1111 ± 1.0208 and 10.8933 ± 1.7411, respectively. Compared with the control group (1.7489 ± 0.9281), the difference was statistically significant (P <0.01). The results of TUNEL showed that the apoptosis of cells in melittin treated group The index of death was significantly higher than that of the control group (P <0.01). Conclusion: Melittin can inhibit U2OS cell proliferation and induce its apoptosis.