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目的探讨咖啡酸苯乙酯(CAPE)对2型糖尿病(T2DM)大鼠肝脏的保护作用及其作用机制。方法 24只雄性SD大鼠随机分为3组,即空白组,损伤组,保护组。高脂高糖饲料喂养联合链脲佐菌素诱导建立2型糖尿病大鼠模型,建模成功后,保护组大鼠CAPE灌胃一个月,测量血清中总胆固醇(TC)和甘油三酯(TG)的含量,并对肝脏中丙二醛(MDA)、蛋白羰基化(PCO)的含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性进行测定。结果损伤组血清中总胆固醇(TC)和甘油三酯(TG)明显上升,说明糖尿病大鼠血脂升高。在损伤组中MDA、PCO含量明显升高,而保护组MDA、PCO的含量较损伤组减少(P<0.05),说明糖尿病能够增加肝脏的氧化应激,诱导氧化损伤,而咖啡酸苯乙酯的保护能够降低MDA、PCO的含量,减少氧化损伤;而SOD、CAT在损伤组中含量降低,可能是机体出现的一种自我保护反应,使得机体本身的抗氧化物质大量消耗,咖啡酸苯乙酯可以减少SOD、CAT含量的增加,保护作用比较明显。结论糖尿病的发生可能增加机体的氧化应激,咖啡酸苯乙酯能够有效降低其氧化损伤程度,从而起到对糖尿病肝脏的保护作用。
Objective To investigate the protective effect of caffeic acid phenethyl ester (CAPE) on the liver of type 2 diabetes mellitus (T2DM) rats and its mechanism. Methods Twenty-four male Sprague-Dawley rats were randomly divided into three groups: blank group, injury group and protection group. The model rats with type 2 diabetes induced by high-fat and high-sugar diet and streptozotocin (STZ) were established. After successful modeling, the rats in protective group were given gavage for one month. The levels of total cholesterol (TC) and triglyceride ), And the content of malondialdehyde (MDA), protein carbonylation (PCO) and activities of superoxide dismutase (SOD) and catalase (CAT) in the liver were measured. Results The levels of total cholesterol (TC) and triglyceride (TG) in the injured group were significantly increased, indicating that the blood lipids in diabetic rats increased. In the injury group, the content of MDA and PCO increased significantly, while the content of MDA and PCO in the protective group decreased compared with the injury group (P <0.05), indicating that diabetes could increase oxidative stress in the liver and induce oxidative damage, while caffeic acid phenethyl ester Can reduce the content of MDA and PCO, and reduce the oxidative damage. However, the content of SOD and CAT in the lesion group is decreased, which may be a kind of self-protection reaction which causes the body to consume a lot of antioxidants, Ester can reduce the increase of SOD, CAT content, the protective effect is more obvious. Conclusion The occurrence of diabetes may increase the body’s oxidative stress. Caffeic acid phenethyl ester can effectively reduce the degree of oxidative damage and thus play a protective role in diabetic liver.