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目的探讨血管内皮生长因子-D(endothelial growth factor-D,VEGF-D)和尿激酶型纤维蛋白酶原激活剂(urokinase-type plasminogen activator,uPA)在结肠癌中的表达特点及临床意义。方法选取30例结肠癌组织及同一段肠管距离肿瘤组织5cm以上的正常组织,分别应用免疫组化S-P法、Western blot方法检测VEGF-D和uPA在结肠癌组织和正常组织中的表达特点,分析它们与临床病理分期之间的关系。结果VEGF-D和uPA在结肠癌组织和正常组织中均有表达,主要表达于细胞质,胞核也有表达,并且在肿瘤组织中的表达明显高于正常组织(P<0.01)。VEGF-D或uPA的表达活性在Dukes C+D期明显高于A+B期(P<0.01),在有淋巴结转移组和远处转移组的表达也高于相应无转移组(P<0.05、P<0.01),随着肿瘤组织分化程度的增高活性依次降低(P<0.05)。结论VEGF-D和uPA在结肠癌组织中的高表达可能在结肠癌的发生发展过程中扮演重要角色。
Objective To investigate the expression and clinical significance of vascular endothelial growth factor-D (VEGF-D) and urokinase-type plasminogen activator (uPA) in colon cancer. Methods 30 cases of colon cancer and the same section of normal tissue more than 5cm away from the tumor were selected. The expression of VEGF-D and uPA in colon cancer tissues and normal tissues were detected by immunohistochemical SP method and Western blot respectively. Their relationship with clinicopathological stages. Results The expression of VEGF-D and uPA in both colon cancer tissues and normal tissues were mainly expressed in cytoplasm and nucleus, and were significantly higher in tumor tissues than in normal tissues (P <0.01). The expression of VEGF-D or uPA in Dukes C + D stage was significantly higher than that in A + B stage (P <0.05), and was higher in patients with lymph node metastasis and distant metastasis than in those without metastasis (P <0.05) , P <0.01). The activity of tumor tissue decreased gradually with the degree of differentiation (P <0.05). Conclusion The high expression of VEGF-D and uPA in colon cancer tissues may play an important role in the development of colon cancer.