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我们利用限制性内切酶BsmI,应用聚合酶链反应(PCR)、限制性片段长度多态性分析(RFLP)技术,测定一组杭州地区汉族正常儿童的维生素D受体(vitaminDreceptor,VDR)基因型和等位基因频率,以期了解中国正常汉族儿童VDR基因多态性分布频?
We used polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) to detect the vitamin D receptor (VDR) gene in normal Han children in Hangzhou area by restriction endonuclease BsmI Type and allele frequencies in order to understand the frequency distribution of VDR gene polymorphism in Chinese normal Han children.