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对用人脑胶质瘤碱性蛋白(GBP)免疫的38只豚鼠和23只对照组豚鼠进行了巨噬细胞电泳试验(MEM),其巨噬细胞电泳减缓率免疫组为6.13±1.06%,对照组为0.96±0.90%;经统计学处理,两组间有显著区别,表明GBP能激发豚鼠的细胞免疫反应性。从三批免疫组和三批对照组豚鼠的脾脏和淋巴结组织中分别提取I-RNA和N-RNA。将49人次健康人的淋巴细胞分别与上述RNA温育,则经I-RNA处理的淋巴细胞能对GBP产生7.73±0.97%的巨噬细胞电泳减缓作用,而经N-RNA处理的仅产生1.29±0.81%的减缓作用,经统计学处理,两组间巨噬细胞电泳减缓率有非常显著的区别,说明GBP免疫组的I-RNA能超越种间界限,使人淋巴细胞获得具有对GBP起免疫反应的能力。
Macrophage electrophoresis (MEM) was performed on 38 guinea pigs immunized with human glioma basic protein (GBP) and 23 control guinea pigs. The rate of decrease of macrophage electrophoresis in the immunized group was 6.13 ± 1.06% Group 0.96 ± 0.90%; statistically significant difference between the two groups, indicating that GBP can stimulate the cellular immune response in guinea pigs. I-RNA and N-RNA were extracted from the spleen and lymph node tissues of guinea pigs in three batches of immunized and three batches of control groups respectively. Lymphocytes from 49 human healthy individuals, respectively, were incubated with the above RNAs, and I-RNA treated lymphocytes produced 7.73 ± 0.97% inhibition of macrophage electrophoresis on GBP, whereas N-RNA treatment produced only 1.29 ± 0.81% of the slow down, the statistical treatment, macrophage electrophoresis between the two groups there is a significant rate of slowdown, indicating that GBP immunization group of I-RNA can transcend species boundaries, so that people with lymphocytes from GBP The ability of the immune response.