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已知脾制剂能提高照射动物的活存率,但大多数作者认为是细胞移植所致,对是否存在体液因素的作用是有争议的。作者用疫苗或内毒素处理的小鼠脾提取物证明了体液因素的存在。本实验使用5周龄的雄性 ICR-JCL 小鼠,注射伤寒疫苗0.5毫升后10～12小时或注射大肠杆菌内毒素10微克后5～6小时,取出小鼠脾脏,将30～40个脾脏收集在一起,制成匀浆,离心后的上清液用 SephadexG-200园柱进行分离。得到的脾提取物Ⅰ～Ⅵ各组份在照后即刻及1～4天给600伦照射
Spleen preparations are known to increase the viability of irradiated animals, but most authors believe this is due to cell transplantation. The role of humoral factors is controversial. The authors used vaccine or endotoxin-treated mouse spleen extract to demonstrate the presence of humoral factors. In this experiment, 5-week-old male ICR-JCL mice were used. Spleen was removed from 10 to 12 hours after injection of typhoid vaccine 0.5 ml and 5 to 6 hours after E. coli endotoxin injection. 30 to 40 spleens were collected Together, they were homogenized and the centrifuged supernatant was separated on a Sephadex G-200 column. The obtained spleen extract Ⅰ ~ Ⅵ each component immediately after irradiation and 1 to 4 days to 600 Lun irradiation