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目的优选大孔吸附树脂分离纯化沙苑子总黄酮(ACSTF)的工艺。方法以ACSTF出膏率、总黄酮质量分数、工艺转移率为指标,采用正交设计法优选水煎煮提取沙苑子的工艺参数、D101型大孔树脂分离纯化ACSTF的工艺参数。结果最优提取工艺为加水煎煮提取3次,每次0.5 h,加水量分别为10、6、6倍药材量。D101树脂分离纯化ACSTF的优化工艺条件为上样液ACSTF质量浓度为约1.0 mg/m L,径高比为1∶5,上样体积流量1 BV/h,比上样量为0.6 g/m L(药材/湿树脂);水洗脱体积流量1 BV/h,水洗脱体积为4 BV;乙醇洗脱体积分数为70%,乙醇洗脱体积流量为1 BV/h,乙醇洗脱体积为5 BV。制成的沙苑子总黄酮提取物出膏率为2.65%,总黄酮质量分数为56.24%,工艺转移率为68.37%。结论该方法简单、可行,能够用来分离纯化ACSTF。
Objective To optimize the process of separation and purification of total flavonoids from Acanthamoeba fasciatus (ACSTF) by macroporous adsorption resin. Methods ACSTF ointment rate, total flavonoids mass fraction, process transfer rate as an indicator, using orthogonal design of water decoction extract Saussurea process parameters, D101 macroporous resin separation and purification of ACSTF process parameters. Results The optimal extraction process was extracting with boiling water for 3 times, each time being 0.5 h. The amount of water added was 10, 6 and 6 times of the amount of medicinal materials respectively. The optimal conditions for the separation and purification of ACSTF from D101 resin were as follows: the mass concentration of ACSTF was about 1.0 mg / m L, the ratio of diameter to height was 1: 5, the volumetric flow rate was 1 BV / h, L (medicinal material / wet resin); water elution volume flow rate 1 BV / h, water elution volume of 4 BV; ethanol elution volume fraction of 70% ethanol elution volume flow rate of 1 BV / h, ethanol elution volume For 5 BV. The total yield of total flavonoids from Aster seeds was 2.65%, the content of total flavonoids was 56.24% and the rate of technology transfer was 68.37%. Conclusion The method is simple and feasible and can be used to separate and purify ACSTF.