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目的探讨黄芩对大黄中5种蒽醌类成分在提取精制过程中相互转化的影响。方法 HPLC测定药材和提取物中5种成分的含量,比较药材和提取物中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的相对比例;再分别用5种蒽醌的对照品加入和不加黄芩药材提取液模拟提取精制过程,考察5种蒽醌成分之间的转化情况。结果大黄药材和提取物中5种成分的相对比例有明显变化,大黄酸在提取物中的比例增高;同时采用对照品模拟提取精制过程时各个成分并无转化,而加入黄芩药材提取液后,大黄酸经过提取精制处理后能部分转化为大黄素,其余4种成分之间无转化。结论黄芩和大黄药材配伍提取可以使大黄蒽醌类成分发生转化。
Objective To study the effects of Scutellaria baicalensis Georgi on transformation of five anthraquinones in rhubarb during the extraction and purification. Methods HPLC method was used to determine the content of five components in medicinal materials and extracts. The relative proportions of aloe-emodin, rhein, emodin, chrysophanol and physcion in medicinal materials and extracts were compared. With and without the addition of Scutellariae Radix extract to simulate the process of extraction and purification, the conversion of five anthraquinones was investigated. Results The relative proportions of five components in rhubarb herbs and extracts were significantly changed, and the proportion of rhein in the extracts was increased. At the same time, the constituents were not transformed when using the reference substance to simulate the extraction and purification process. However, after adding Scutellariae Radix extract, After extraction and purification, rhein can be partially converted into emodin, and the remaining four components are not transformed. Conclusion Scutellaria and rhubarb compatibility extraction can make rhubarb anthraquinone components transformation.