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采用镉还原柱层析和比色法测定了１７例哮喘患儿血浆亚硝酸／硝酸根离子（ＮＯ－２／ＮＯ－３）水平，另用生物学活性法测定了肿瘤坏死因子（ＴＮＦ）及丙二醛（ＭＤＡ）和超氧化物歧化酶（ＳＯＤ）水平。结果：小儿哮喘发作期血浆ＮＯ－２／ＮＯ－３、ＭＤＡ及ＴＮＦ水平明显升高（Ｐ分别＜０．０５或００１）；在缓解期ＮＯ－２／ＮＯ－３和ＭＤＡ水平恢复正常（Ｐ＞０．０５），ＴＮＦ水平虽有明显降低，但仍高于对照组（Ｐ＜０．０１）；而ＳＯＤ水平在急性发作期明显降低（Ｐ＜０．０１），缓解期恢复正常（Ｐ＞０．０５）。提示：哮喘发作时不仅有氧自由基增多，而且还有一氧化氮自由基增加，它们相互作用，共同损伤气道上皮等肺组织，加重炎症反应，导致气道高反应性而引起和（或）加重哮喘发病
Plasma nitrous acid / nitrate ion (NO-2 / NO-3) levels were measured in 17 asthmatic children by cadmium reduction column chromatography and colorimetric assay. The levels of tumor necrosis factor (TNF) and tumor necrosis factor Malondialdehyde (MDA) and superoxide dismutase (SOD) levels. Results: The levels of plasma NO-2 / NO-3, MDA and TNF were significantly increased in children with asthma attack (P <0.05 or 001, respectively). The levels of NO-2 / (P> 0.05). The level of TNF was significantly lower than that of the control group (P <0.01), while the level of SOD was significantly decreased at acute episode (P <0.01) and returned to normal at remission (P> 0.05). Tip: not only the increase of oxygen free radicals during asthma attack, but also increase the free radicals of nitric oxide, they interact, damage airway epithelium and other lung tissues together, aggravating the inflammatory response, leading to airway hyperresponsiveness caused and / Increased asthma incidence