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研究了在液体木屑培养基中添加0.005、0.01、0.015 mg/mL浓度的氨基寡糖对3株侧耳发酵液中漆酶活性的影响,结果表明:氨基寡糖对侧耳漆酶的产生有明显地促进作用,3株侧耳菌株在氨基寡糖液体木屑培养基中均有漆酶活性,酶活随发酵时间和氨基寡糖浓度而变化,冀微961糙皮侧耳在0.01 mg/mL氨基寡糖浓度,第10 d时漆酶酶活达到最高238 U/mL,比对照增加49.7%。漆酶参与木质素的降解,对木腐菌的生长有着直接的影响,氨基寡糖通过促进漆酶活性,影响侧耳的生长发育。
The effects of adding oligosaccharides with concentrations of 0.005, 0.01 and 0.015 mg / mL on the laccase activity of three strains of Pleurotus ferulae were studied. The results showed that the production of laccase The results showed that the three strains of Pleurotus ostreatus all had laccase activity in the liquid oligosaccharides liquid woodruffs, and the enzyme activity varied with the fermentation time and the concentration of amino oligosaccharides. , Laccase activity reached the maximum of 238 U / mL on the 10th day, an increase of 49.7% compared with the control. Laccases are involved in the degradation of lignin and have a direct impact on the growth of wood-rot fungi. Amino-oligosaccharides affect the growth and development of Pleurotus ostreatus through the promotion of laccase activity.