,Rapid and efficient CRISPR-mediated genome editing with cloning-free method

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The CRISPR/Cas9 system evolved as a self-defense mechanism for bacteria and archaea [1].Based on a small customizable guide RNA and the Cas9 nuclease,it has now been retooled asmolecule-sized programmable scissors,which can precisely cleave target at any desired position in multispecies genome.It holds great hope for precise therapeutics for human diseases [2,3].
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