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目的:检测结肠腺癌中微小RNA(microRNA，miR)-134-5p的表达，分析其与细胞增殖的关联性，探讨其与肝转移的关系。方法:2013年4月至2014年8月，收集大连大学附属中山医院确诊为结肠腺癌并行手术治疗的患者共69例作为研究对象，选取肿瘤组织作为观察组，选取距肿瘤的边缘>5 cm的正常结肠组织作为对照组。应用实时荧光定量聚合酶链反应(PCR)法检测二组中miR-134-5p的表达。应用免疫组织化学法检测观察组中增殖细胞核抗原(PCNA)的表达，组间比较采用n t检验。n 结果:miR-134-5p在观察组中的表达明显低于对照组(1.15±0.38比1.55±0.42，n t＝6.670，n P<0.01)，差异有统计学意义，miR-134-5p的表达在不同肿物最大径(1.01±0.26比1.35±0.25，n χ2＝3.110，n P<0.05)、有无脉管累犯(0.85±0.25比1.32±0.27，n χ2＝4.990，n P<0.05)、不同浸润深度(1.02±0.30比1.35±0.31，n χ2＝3.040，n P<0.05)、有无淋巴结转移(0.95±0.22比1.28±0.40，n χ2＝3.540，n P<0.05)和不同TNM分期(0.98±0.29比1.36±0.28，n χ2＝3.980，n P<0.05)的分组中差异均有统计学意义。相关分析显示miR-134-5p与PCNA呈负相关(n r＝-0.560，n P<0.05)。随访中肝转移者miR-134-5p的表达低于无肝转移者(0.78±0.11比1.44±0.32，n t＝6.950，n P<0.05)，随访显示miR-134-5p的表达与生存时间相关(n χ2＝4.330，n P<0.05)，差异均有统计学意义。n 结论:miR-134-5p在结肠腺癌组织中低表达，对肿瘤的形成和进展有重要作用，miR-134-5p异常表达时可能对细胞增殖有促进作用。miR-134-5p的表达可能与预后有关。“,”Objective:To detect the expression of microRNA (miRNA, miR)-134-5p in colorectal adenocarcinoma, and explore its relationship with cell proliferation and liver metastasis.Methods:Totally 69 patients with colorectal adenocarcinoma in Affiliated Zhongshan Hospital of Dalian University were selected as the study subjects from April 2013 to August 2014. Tumor tissue was selected as the observation group, and normal colorectal mucosa tissue was selected as the control group. Real-time fluorescence quantitative polymerase chain reaction (PCR) was used to detect the expression of miR-134-5p in the two groups. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemical method in the observation group.Results:The expression of miR-134-5p was significantly lower in the observation group than that in the control group (1.15±0.38 vs. 1.55±0.42, n t=6.67, n P<0.01). The expression of miR-134-5p was significantly different in maximum diameter (1.01±0.26 vs. 1.35±0.25,n χ2=3.11, n P<0.05), vascular recidivism (0.85±0.25 vs. 1.32±0.27,n χ2=4.99, n P<0.05), depth of invasion (1.02±0.30 vs. 1.35±0.31,n χ2=3.04, n P<0.05), lymph node metastasis (0.95±0.22 vs. 1.28±0.40,n χ2=3.54, n P<0.05) and TNM stage (0.98±0.29 vs. 1.36±0.28,n χ2=3.98, n P<0.05) in the observation group. The correlation analysis showed that there was a negative correlation between miR-134-5p and PCNA (n r=-0.56, n P<0.05). During follow-up, the expression of miR-134-5p was significantly lower in liver metastasis patients than that in those without liver metastasis (0.78±0.11 vs. 1.44±0.32,n t=6.95, n P<0.05). During the follow-up period, the expression of miR-134-5p was related to survival time (n χ2=4.33, n P<0.05).n Conclusion:The low expression of miR-134-5p plays an important role in the formation and progression of colorectal adenocarcinoma. The abnormal expression of miR-134-5p may promote cell proliferation. The expression of miR-134-5p may be related to prognosis. The low expression of miR-134-5p may be valuable in predicting liver metastasis.