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用杂交瘤技术建立的一株分泌抗人精浆蛋白的使人精子制动单抗的细胞株，Ｉｇ亚类分析表明，此单抗为ＩｇＭ。间接ＥＬＩＳＡ试验、精子制动试验、精子凝集试验和间接免疫荧光等试验分别证明所得单抗能与人精浆和无精子症患者的精浆产生特异免疫反应，有制动人精子的作用，并能特异结合到人精子的颈部，从而证实此单抗是精子制动单抗，其相应的抗原系精浆和精子共有的精子膜抗原，用ＦＰＬＣ－羟基磷灰石柱层析成功地从小鼠腹水纯化了此单抗。
A monoclonal antibody against human seminiferous stem cells secreting human anti-human seminal plasma was subclassified by hybridoma technique. Ig analysis showed that the McAb was IgM. Indirect ELISA test, sperm brake test, sperm agglutination test and indirect immunofluorescence test showed that the resulting McAb can produce specific immune response with seminal plasma of patients with seminal plasma and azoospermia, and have the effect of braking sperm Can specifically bind to the human sperm neck, thus confirming that the monoclonal antibody is sperm brake monoclonal antibody, the corresponding antigen seminal plasma and sperm membrane antigen common sperm, FPLC-hydroxyapatite column chromatography successfully from childhood Murine ascites purified this mAb.