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本实验将人类正常G6PDcDNA与逆转录病毒载体 pLXSN重组获得表达载体pLG6PDSN ,通过脂质体介导法转染红白血病细胞系K5 6 2 ,G418筛选阳性克隆细胞并测G6PD表达。该表达载体的构建成功为严重G6PD缺陷症的基因治疗奠定了基础。
In this experiment, the human normal G6PD cDNA was recombined with the retroviral vector pLXSN to obtain the expression vector pLG6PDSN. The erythroleukemia cell line K562 was transfected by lipofectamine and the positive clones were screened by G418 and the expression of G6PD was measured. The successful construction of this expression vector lays the foundation for the gene therapy of severe G6PD deficiency.