体外LAK细胞与人肺癌细胞接触的电镜观察

• 作 者: 史家岚 朱平 王吾如 徐震逸 张月桂 巴德年
• 机 构: 哈尔滨医科大学病理学教研室,哈尔滨医科大学电镜室,哈尔滨医科大学病理学教研室,哈尔滨医科大学肿瘤研究所免疫室,哈尔滨医科大学肿瘤研究所免疫室,哈尔滨医科大学肿瘤研究所免疫室
• 出 处: 哈尔滨医科大学学报
• 发表日期: 1988年06期
• 关键词: 人肺癌细胞 LAK 戊二醛 靶细胞 体外培养 锇酸 孔板 白细胞介素 酒精 树脂

体内、体外研究证明,LAK细胞具有抗肿瘤作用,但从形态学上来研究LAK细胞杀伤机理的报道很少。本研究用96孔板培养的人肺癌细胞作为靶细胞。将由白细胞介素-2激活的、体外培养6天具有LAK样活性的细胞与贴壁生长的靶细胞共同孵育10分钟、1小时、2小时、4小时后,用2.5%戊二醛及1%锇酸原位双重固定,逐级酒精脱水、树脂812包埋,

In vivo and in vitro studies have shown that LAK cells have anti-tumor effects, but few reports of the mechanism of LAK cell killing. In this study, human lung cancer cells cultured in 96-well plates were used as target cells. Cells that had been activated by interleukin-2 and had LAK-like activity for 6 days in vitro were incubated with adherently growing target cells for 10 minutes, 1 hour, 2 hours and 4 hours later with 2.5% glutaraldehyde and 1% Osmium tetroxide in situ double fixed, gradual alcohol dehydration, resin embedded in 812,